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Literature summary extracted from

  • Arroyo, M.; Menendez, M.; Garcia, J.L.; Campillo, N.; Hormigo, D.; De la Mata, I.; Castillon, M.P.; Acebal, C.
    The role of cofactor binding in tryptophan accessibility and conformational stability of His-tagged D-amino acid oxidase from Trigonopsis variabilis (2007), Biochim. Biophys. Acta, 1774, 556-565.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 the His-tagged enzyme is expressed in Escherichia coli Trigonopsis variabilis

General Stability

EC Number General Stability Organism
1.4.3.3 His-tagged DAAO loses its flavin cofactor upon dilution or prolonged dialysis against FAD-free solvents leading to the catalytic inactivation Trigonopsis variabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 5.5
-
 D-alanine apparent value, reconstituted holoenzyme at pH 8.0 and 30°C Trigonopsis variabilis

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Trigonopsis variabilis Q99042
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3
-
 Trigonopsis variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 cephalosporin C + H2O + O2
-
 Trigonopsis variabilis 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-alanine + H2O + O2
-
 Trigonopsis variabilis pyruvate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.3 dimer
-
 Trigonopsis variabilis

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Trigonopsis variabilis
1.4.3.3 DAAO
-
 Trigonopsis variabilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 30 45 loss of secondary structure and inactivation occurs over a range of 30°C to 45°C, the melting temperature is at 41.4°C Trigonopsis variabilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 95
-
 D-alanine apparent value, reconstituted holoenzyme at pH 8.0 and 30°C Trigonopsis variabilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD contains non-covalently bound FAD Trigonopsis variabilis