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Literature summary extracted from

  • Zeng, J.; Liu, Y.; Wu, L.; Li, D.
    Mutation of Tyr375 to Lys375 allows medium-chain acyl-CoA dehydrogenase to acquire acyl-CoA oxidase activity (2007), Biochim. Biophys. Acta, 1774, 1628-1634.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.6 expressed in Escherichia coli BL21(DE3) cells Rattus norvegicus
1.3.8.7 expressed in Escherichia coli BL21(DE3) cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.7 Y375A very low acyl-CoA dehydrogenase activity Rattus norvegicus
1.3.8.7 Y375E inactive Rattus norvegicus
1.3.8.7 Y375F very low acyl-CoA dehydrogenase activity Rattus norvegicus
1.3.8.7 Y375K very low acyl-CoA dehydrogenase activity, mutant exhibits acyl-CoA oxidase activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.6 0.03
-
 hexanoyl-CoA in 50 mM phosphate buffer, pH 7.4, at 30°C Rattus norvegicus
1.3.8.7 0.00084
-
 octanoyl-CoA mutant enzyme Y375K, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
1.3.8.7 0.0023
-
 octanoyl-CoA wild type enzyme, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
1.3.8.7 0.00242
-
 octanoyl-CoA mutant enzyme Y375F, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
1.3.8.7 0.00684
-
 octanoyl-CoA mutant enzyme Y375A, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.8.7 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.6 45000
-
 SDS-PAGE Rattus norvegicus
1.3.8.7 45000
-
 SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.6 Rattus norvegicus
-
-
-
1.3.8.7 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.6 nickel metal-affinity resin column chromatography Rattus norvegicus
1.3.8.7 HiTrap chelating nickel affinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.6 liver
-
 Rattus norvegicus
-
1.3.8.7 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.3.8.7 -80°C, 6 months, remains stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.6 hexanoyl-CoA + O2
-
 Rattus norvegicus (2E)-hex-2-enoyl-CoA + H2O2
-
 ?
1.3.8.7 acyl-CoA + acceptor
-
 Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
 ?
1.3.8.7 hexanoyl-CoA + 2,6-dichloroindophenol
-
 Rattus norvegicus 2-hexenoyl-CoA + reduced 2,6-dichloroindophenol
-
 ?
1.3.8.7 octanoyl-CoA + 2,6-dichloroindophenol optimal substrate for wild type enzyme Rattus norvegicus 2-octenoyl-CoA + reduced 2,6-dichloroindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.6 ACO I
-
 Rattus norvegicus
1.3.3.6 MCAD Y375K Y375K mutant of medium-chain acyl-CoA dehydrogenase that exhibits acyl-CoA oxidase activity Rattus norvegicus
1.3.8.7 MCAD
-
 Rattus norvegicus
1.3.8.7 medium chain acyl-CoA dehydrogenase
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.6 0.089
-
 hexanoyl-CoA in 50 mM phosphate buffer, pH 7.4, at 30°C Rattus norvegicus
1.3.8.7 0.038
-
 octanoyl-CoA mutant enzyme Y375K, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
1.3.8.7 0.22
-
 octanoyl-CoA mutant enzyme Y375A, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
1.3.8.7 0.37
-
 octanoyl-CoA mutant enzyme Y375F, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
1.3.8.7 3.3
-
 octanoyl-CoA wild type enzyme, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.6 FAD
-
 Rattus norvegicus
1.3.8.7 FAD
-
 Rattus norvegicus