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Literature summary extracted from

  • Ivarsson, Y.; Norrgard, M.A.; Hellman, U.; Mannervik, B.
    Engineering the enantioselectivity of glutathione transferase by combined active-site mutations and chemical modifications (2007), Biochim. Biophys. Acta, 1770, 1374-1381.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 C115A mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens
2.5.1.18 C174A mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens
2.5.1.18 C78A mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens
2.5.1.18 C88A mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens
2.5.1.18 G112C mutant displays as high catalytic activity as wild type GST M1-1 Homo sapiens
2.5.1.18 I10A/G112C mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens
2.5.1.18 I10C mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.035
-
 (R,R)-trans-stilbene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.06
-
 (R,R)-trans-stilbene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.08
-
 (S,S)-trans-stilbene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.1
-
 (R,R)-trans-stilbene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.11
-
 (S,S)-trans-stilbene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.12
-
 (S,S)-trans-stilbene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.8
-
 (R,R)-1-phenylpropylene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.9
-
 (R,R)-1-phenylpropylene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 1.3
-
 (S)-styrene-7,8-oxide mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 1.6
-
 (S)-styrene-7,8-oxide mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 2.2
-
 (S,S)-1-phenylpropylene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 2.4
-
 (S,S)-1-phenylpropylene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 2.4
-
 (R,R)-1-phenylpropylene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 3.8
-
 (S,S)-1-phenylpropylene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 4.2
-
 (R)-styrene-7,8-oxide mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 4.7
-
 (R)-styrene-7,8-oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 4.9
-
 (S)-styrene-7,8-oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 8.8
-
 (R)-styrene-7,8-oxide mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 GSH-affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + (R)-styrene-7,8-oxide
-
 Homo sapiens ?
-
 ?
2.5.1.18 glutathione + (R,R)-1-phenylpropylene oxide
-
 Homo sapiens ?
-
 ?
2.5.1.18 glutathione + (R,R)-trans-stilbene oxide
-
 Homo sapiens ?
-
 ?
2.5.1.18 glutathione + (S)-styrene-7,8-oxide
-
 Homo sapiens ?
-
 ?
2.5.1.18 glutathione + (S,S)-1-phenylpropylene oxide
-
 Homo sapiens ?
-
 ?
2.5.1.18 glutathione + (S,S)-trans-stilbene oxide
-
 Homo sapiens ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Homo sapiens S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 homodimer x-ray crystallography Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione transferase M1-1
-
 Homo sapiens
2.5.1.18 GST M1-1 originally called GST mu Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.07
-
 (S,S)-trans-stilbene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.2
-
 (S,S)-trans-stilbene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.37
-
 (R,R)-trans-stilbene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.46
-
 (S,S)-trans-stilbene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 0.83
-
 (R,R)-1-phenylpropylene oxide mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 1.1
-
 (S,S)-1-phenylpropylene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 1.3
-
 (R,R)-trans-stilbene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 2.3
-
 (S)-styrene-7,8-oxide mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 2.4
-
 (S,S)-1-phenylpropylene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 2.7
-
 (R)-styrene-7,8-oxide mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 3.2
-
 (S)-styrene-7,8-oxide mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 4.4
-
 (R)-styrene-7,8-oxide mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 4.6
-
 (R,R)-1-phenylpropylene oxide mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 4.8
-
 (R)-styrene-7,8-oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 7.7
-
 (R,R)-trans-stilbene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 8.4
-
 (S)-styrene-7,8-oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 9
-
 (S,S)-1-phenylpropylene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.5.1.18 17.7
-
 (R,R)-1-phenylpropylene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens