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Literature summary extracted from

  • Occhipinti, E.; Bec, N.; Gambirasio, B.; Baietta, G.; Martelli, P.L.; Casadio, R.; Balny, C.; Lange, R.; Tortora, P.
    Pressure and temperature as tools for investigating the role of individual non-covalent interactions in enzymatic reactions Sulfolobus solfataricus carboxypeptidase as a model enzyme (2006), Biochim. Biophys. Acta, 1764, 563-572.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.17.B1 additional information effect of temperature and pressure on conformational modifications and enzyme-substrate interactions are generally dissimilar and barely predictable Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.B1 expressed as an N-terminus His-tagged protein in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.B1 0.005
-
 L-Bz-His at pH 8.0 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.013
-
 L-Fur-Phe at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.014
-
 L-Bz-Ala at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.014
-
 L-Bz-Arg at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.019
-
 L-Bz-Glu at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.021
-
 L-Bz-His at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.028
-
 L-Bz-Met at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.028
-
 Fur-Gly at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.034
-
 L-Bz-Phe at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.043
-
 L-Bz-Phe at pH 8.0 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.061
-
 Bz-Gly at pH 8.0 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.103
-
 L-Fur-Leu at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.143
-
 Bz-Gly at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.144
-
 L-Bz-Phe at pH 5.7 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.149
-
 Bz-Gly at pH 5.7 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.181
-
 L-Bz-His at pH 5.7 and 35°C Saccharolobus solfataricus
3.4.17.B1 0.501
-
 L-Bz-Leu at pH 6.5 and 35°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.B1 Zn2+
-
 Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.B1 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.B1 by affinity chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.B1 Bz-Gly + H2O
-
 Saccharolobus solfataricus benzoic acid + glycine
-
 ?
3.4.17.B1 Fur-Gly + H2O
-
 Saccharolobus solfataricus furoic acid + glycine
-
 ?
3.4.17.B1 L-Bz-Ala + H2O
-
 Saccharolobus solfataricus benzoic acid + L-alanine
-
 ?
3.4.17.B1 L-Bz-Arg + H2O
-
 Saccharolobus solfataricus benzoic acid + L-arginine
-
 ?
3.4.17.B1 L-Bz-Glu + H2O
-
 Saccharolobus solfataricus benzoic acid + L-glutamate
-
 ?
3.4.17.B1 L-Bz-His + H2O
-
 Saccharolobus solfataricus benzoic acid + L-histidine
-
 ?
3.4.17.B1 L-Bz-Leu + H2O
-
 Saccharolobus solfataricus benzoic acid + L-leucine
-
 ?
3.4.17.B1 L-Bz-Met + H2O
-
 Saccharolobus solfataricus benzoic acid + L-methionine
-
 ?
3.4.17.B1 L-Bz-Phe + H2O
-
 Saccharolobus solfataricus benzoic acid + L-phenylalanine
-
 ?
3.4.17.B1 L-Fur-Leu + H2O
-
 Saccharolobus solfataricus furoic acid + L-leucine
-
 ?
3.4.17.B1 L-Fur-Phe + H2O
-
 Saccharolobus solfataricus furoic acid + L-phenylalanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.B1 CPS
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.17.B1 35 65
-
 Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.B1 1.03
-
 Bz-Gly at pH 5.7 and 35°C Saccharolobus solfataricus
3.4.17.B1 1.65
-
 Bz-Gly at pH 8.0 and 35°C Saccharolobus solfataricus
3.4.17.B1 2.05
-
 L-Bz-His at pH 8.0 and 35°C Saccharolobus solfataricus
3.4.17.B1 2.3
-
 Fur-Gly at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 2.8
-
 L-Bz-Ala at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 2.95
-
 L-Bz-Met at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 3.25
-
 L-Fur-Phe at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 3.58
-
 L-Fur-Leu at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 3.63
-
 L-Bz-Arg at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 4.15
-
 Bz-Gly at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 4.3
-
 L-Bz-Glu at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 4.3
-
 L-Bz-Phe at pH 8.0 and 35°C Saccharolobus solfataricus
3.4.17.B1 4.57
-
 L-Bz-Phe at pH 5.7 and 35°C Saccharolobus solfataricus
3.4.17.B1 4.87
-
 L-Bz-Phe at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 7.77
-
 L-Bz-His at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 8.35
-
 L-Bz-Leu at pH 6.5 and 35°C Saccharolobus solfataricus
3.4.17.B1 8.83
-
 L-Bz-His at pH 5.7 and 35°C Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.17.B1 5.7 8
-
 Saccharolobus solfataricus