EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.304 | mutant enzymes expressed in Escherichia coli | Cupriavidus necator |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.304 | A372_C382del | no detectable activity | Cupriavidus necator |
2.3.1.304 | C438G | no detectable activity | Cupriavidus necator |
2.3.1.304 | D281_D290del | no detectable activity | Cupriavidus necator |
2.3.1.304 | E267K | 40% of wild-type activity | Cupriavidus necator |
2.3.1.304 | E578_A589del | no detectable activity | Cupriavidus necator |
2.3.1.304 | L446K | 15% of wild-type activity, no change in substrate specificity | Cupriavidus necator |
2.3.1.304 | additional information | fusion proteins composed of an N-terminal class II PHA synthase region (PhaCPa from Pseudomonas aeruginosa) and a C-terminal class I PHA synthase region (PhaCRe from Ralstonia eutropha) are constructed | Cupriavidus necator |
2.3.1.304 | T231I | no detectable activity | Cupriavidus necator |
2.3.1.304 | T323S | no detectable activity | Cupriavidus necator |
2.3.1.304 | V585_A589del | no detectable activity | Cupriavidus necator |
2.3.1.304 | Y445F | 38% of wild-type activity, no change in substrate specificity | Cupriavidus necator |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.304 | Cupriavidus necator | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.304 | (R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | - |
Cupriavidus necator | [(R)-3-hydroxybutanoate](n+1) + CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.304 | class I PHA synthase | - |
Cupriavidus necator |
2.3.1.304 | poly(3-hydroxybutyrate) synthase | - |
Cupriavidus necator |