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Literature summary extracted from

  • Rehm, B.H.; Antonio, R.V.; Spiekermann, P.; Amara, A.A.; Steinbuchel, A.
    Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: in vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model (2002), Biochim. Biophys. Acta, 1594, 178-190.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 mutant enzymes expressed in Escherichia coli Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.304 A372_C382del no detectable activity Cupriavidus necator
2.3.1.304 C438G no detectable activity Cupriavidus necator
2.3.1.304 D281_D290del no detectable activity Cupriavidus necator
2.3.1.304 E267K 40% of wild-type activity Cupriavidus necator
2.3.1.304 E578_A589del no detectable activity Cupriavidus necator
2.3.1.304 L446K 15% of wild-type activity, no change in substrate specificity Cupriavidus necator
2.3.1.304 additional information fusion proteins composed of an N-terminal class II PHA synthase region (PhaCPa from Pseudomonas aeruginosa) and a C-terminal class I PHA synthase region (PhaCRe from Ralstonia eutropha) are constructed Cupriavidus necator
2.3.1.304 T231I no detectable activity Cupriavidus necator
2.3.1.304 T323S no detectable activity Cupriavidus necator
2.3.1.304 V585_A589del no detectable activity Cupriavidus necator
2.3.1.304 Y445F 38% of wild-type activity, no change in substrate specificity Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Cupriavidus necator
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 (R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
Cupriavidus necator [(R)-3-hydroxybutanoate](n+1) + CoA
-
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Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 class I PHA synthase
-
Cupriavidus necator
2.3.1.304 poly(3-hydroxybutyrate) synthase
-
Cupriavidus necator