Literature summary extracted from

Tsygankov, A.A.; Minakov, E.A.; Zorin, N.A.; Gosteva, K.S.; Voronin, O.G.; Karyakin, A.A.
Measuring the pH dependence of hydrogenase activities (2007), Biochemistry, 72, 968-973.

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.12.99.6	Desulfomicrobium baculatum	-	-	-
1.12.99.6	Desulfomicrobium baculatum 9974	-	-	-
1.12.99.6	Thiocapsa roseopersicina	-	-	-
1.12.99.6	Thiocapsa roseopersicina Bbs	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.12.99.6	DEAE-cellulose column chromatography and phenyl-Sepharose column chromatography	Desulfomicrobium baculatum

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.12.99.6	H2 + oxidized benzyl viologen	-	Desulfomicrobium baculatum	H+ + reduceded benzyl viologen	-	r
1.12.99.6	H2 + oxidized benzyl viologen	-	Thiocapsa roseopersicina	H+ + reduceded benzyl viologen	-	r
1.12.99.6	H2 + oxidized benzyl viologen	-	Thiocapsa roseopersicina Bbs	H+ + reduceded benzyl viologen	-	r
1.12.99.6	H2 + oxidized benzyl viologen	-	Desulfomicrobium baculatum 9974	H+ + reduceded benzyl viologen	-	r

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.12.99.6	5.5	6.5	the steady-state current caused by catalytic uptake of hydrogen is maximal at pH 5.5-6.5 under overvoltage of 30-60 mV	Thiocapsa roseopersicina
1.12.99.6	6	6.5	the steady-state current caused by catalytic uptake of hydrogen is maximal at pH 6.0-6.5 under overvoltage of 30-60 mV	Desulfomicrobium baculatum
1.12.99.6	8.5	-	-	Desulfomicrobium baculatum
1.12.99.6	9.5	-	in solution	Thiocapsa roseopersicina

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Release 2023.1 (January 2023)