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Literature summary extracted from

  • Faucher, F.; Cantin, L.; Luu-The, V.; Labrie, F.; Breton, R.
    The crystal structure of human DELTA4-3-ketosteroid 5beta-reductase defines the functional role of the residues of the catalytic tetrad in the steroid double bond reduction mechanism (2008), Biochemistry, 47, 8261-8270.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.3 expressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.3 hanging drop vapour diffusion method in 20% (w/v) PEG-4K, 0.1 M sodium cacodylate (pH 6.5), 0.2 M sodium citrate, 0.5 M (NH4)2SO4 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.3 65000
-
 recombinant GST-5beta-reductase fusion protein, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Homo sapiens P51857
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.3 glutathione-Sepharose column chromatography and Sephacryl-S100 gel filtration Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.3 17,21-dihydroxy-5beta-pregnane-3,11,20-trione + NADP+ = cortisone + NADPH + H+ catalyzes the reduction of the DELTA4 double bond of DELTA4-3-ketosteroids, converting those steroids into 5beta-dihydro-3-ketosteroids Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 7alpha,12beta-dihydroxy-4-cholesten-3-one + NADPH + H+
-
 Homo sapiens (5beta,7alpha,12beta)-7,12-dihydroxycholestan-3-one + NADP+
-
 ?
1.3.1.3 7alpha-hydroxy-4-cholesten-3-one + NADPH
-
 Homo sapiens (5beta,7alpha)-7-hydroxycholestan-3-one + NADP+
-
 ?
1.3.1.3 progesterone + NADPH + H+
-
 Homo sapiens pregnan-3,20-dione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.3 5beta-red
-
 Homo sapiens
1.3.1.3 DELTA4-3-ketosteroid 5beta-reductase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.3 NADPH
-
 Homo sapiens