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Literature summary extracted from
- 13C NMR characterization of an exchange reaction between CO and CO2 catalyzed by carbon monoxide dehydrogenase (2008), Biochemistry, 47, 6770-6781.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.2.7.4 | expressed in Escherichia coli BL21(DE3) cells | Carboxydothermus hydrogenoformans |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.2.7.4 | C56A | devoid of CO oxidation activity | Carboxydothermus hydrogenoformans |
| 1.2.7.4 | K563L | very low CO oxidation activity | Carboxydothermus hydrogenoformans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.7.4 | cyanide | inhibitory at 5 mM; strong competitive inhibitor | Carboxydothermus hydrogenoformans |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.7.4 | 0.018 | - |
CO | at 70°C | Carboxydothermus hydrogenoformans | |
| 1.2.7.4 | 4 | - |
methyl viologen | at 70°C | Carboxydothermus hydrogenoformans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.7.4 | Fe2+ | contains 9 Fe+ per monomer | Carboxydothermus hydrogenoformans | |
| 1.2.7.4 | Ni2+ | contains 0.1 Ni2+ per monomer | Carboxydothermus hydrogenoformans | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.7.4 | Carboxydothermus hydrogenoformans | - |
strain DSMZ 6008 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.7.4 | Ni-NTA agarose column chromatography | Carboxydothermus hydrogenoformans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.7.4 | CO + H2O + acceptor | - |
Carboxydothermus hydrogenoformans | CO2 + reduced acceptor | - |
? | |
| 1.2.7.4 | CO + H2O + methyl viologen | - |
Carboxydothermus hydrogenoformans | CO2 + reduced methyl viologen | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.7.4 | CODH-II | - |
Carboxydothermus hydrogenoformans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.7.4 | 1500 | - |
CO | at 20°C | Carboxydothermus hydrogenoformans | |
| 1.2.7.4 | 15900 | - |
CO | at 70°C | Carboxydothermus hydrogenoformans | |
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Release 2023.1 (January 2023)
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