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Literature summary extracted from

  • White, T.A.; Johnson, W.H.; Whitman, C.P.; Tanner, J.J.
    Structural basis for the inactivation of Thermus thermophilus proline dehydrogenase by N-propargylglycine (2008), Biochemistry, 47, 5573-5580.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.B2
-
Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.99.B2 sitting drop vapour diffusion method, with 100 mM imidazole buffer at pH 7, 100 mM MgCl2, 14% 2-methyl-2,4-pentanediol, and 5 mM dithiothreitol Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.99.B2 N-propargylglycine
-
Thermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.99.B2 mitochondrion
-
Thermus thermophilus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.99.B2 37980
-
native enzyme, calculated from sequence of cDNA Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.B2 Thermus thermophilus Q72IB8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.B2
-
Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O
-
Thermus thermophilus (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
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Synonyms

EC Number Synonyms Comment Organism
1.5.99.B2 PRODH
-
Thermus thermophilus
1.5.99.B2 proline dehydrogenase
-
Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.B2 FAD
-
Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.99.B2 0.2
-
N-propargylglycine at 25°C Thermus thermophilus