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Literature summary extracted from

  • Doukov, T.I.; Blasiak, L.C.; Seravalli, J.; Ragsdale, S.W.; Drennan, C.L.
    Xenon in and at the end of the tunnel of bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase (2008), Biochemistry, 47, 3474-3483.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.7.4
-
Moorella thermoacetica
2.3.1.169 a 2.5 A resolution structure of xenon-pressurized CODH/ACS, examination of the nature of gaseous cavities within the enzyme. The cavity calculation program CAVENV accurately predicts the channels connecting the C- and A-clusters, with 17 of 19 xenon binding sites within the predicted regions. The enzyme has a channel for a small substrate, a channel plug, a flexible acetyl-CoA synthase subunit that can open to interact with a large substrate, and an interdomain cavity to putatively bind a medium-sized substrate Moorella thermoacetica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.7.4 mitochondrion
-
Moorella thermoacetica 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.4 Ni-Fe-S center
-
Moorella thermoacetica
6.2.1.1 Fe2+ CODH/ACS uses a Ni-Fe-S center called the C-cluster to reduce carbon dioxide to carbon monoxide and uses a second Ni-Fe-S center, called the A-cluster, to assemble acetyl-CoA from a methyl group, coenzyme A, and C-cluster-generated CO Moorella thermoacetica
6.2.1.1 additional information the enzyme uses seven metalloclusters in four reaction steps, overview Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.1 310000
-
-
Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.1 additional information Moorella thermoacetica CODH/ACS is a bifunctional enzyme that is responsible for the reduction of CO2 to CO and subsequent assembly of acetyl-CoA, as part of the Wood-Ljungdahl carbon fixation pathway, the enzyme is a key player in the global carbon cycle ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Moorella thermoacetica
-
formerly Clostridium thermoaceticum strain ATCC 39073
-
2.3.1.169 Moorella thermoacetica
-
bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase
-
6.2.1.1 Moorella thermoacetica
-
formerly Clostridium thermoaceticum strain ATCC 39073
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.4
-
Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + acceptor
-
Moorella thermoacetica CO2 + reduced acceptor
-
?
1.2.7.4 CO + H2O + acceptor
-
Moorella thermoacetica CO2 + reduced acceptor
-
r
6.2.1.1 additional information CODH/ACS is a bifunctional enzyme that is responsible for the reduction of CO2 to CO and subsequent assembly of acetyl-CoA, as part of the Wood-Ljungdahl carbon fixation pathway, the enzyme is a key player in the global carbon cycle Moorella thermoacetica ?
-
?
6.2.1.1 additional information the enzyme is a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase, Moorella thermoacetica CODH/ACS contains a very long enzyme channel to allow for intermolecular CO transport, mechanism and reaction steps, overview. Structure-function analysis in comparison to monofunctional Acs, overview Moorella thermoacetica ?
-
?

Subunits

EC Number Subunits Comment Organism
1.2.7.4 heterotetramer x-ray crystallography Moorella thermoacetica
6.2.1.1 More Moorella thermoacetica CODH/ACS contains a very long enzyme channel to allow for intermolecular CO transport, overview Moorella thermoacetica
6.2.1.1 tetramer alpha2beta2 Moorella thermoacetica

Synonyms

EC Number Synonyms Comment Organism
1.2.7.4 carbon monoxide dehydrogenase/acetyl-CoA synthase bifunctional enzyme Moorella thermoacetica
1.2.7.4 CODH/ACS
-
Moorella thermoacetica
1.2.7.4 CODH/ACS bifunctional enzyme carbon monoxide dehydrogenase/acetyl-CoA synthase Moorella thermoacetica
6.2.1.1 carbon monoxide dehydrogenase/acetyl-CoA synthase
-
Moorella thermoacetica
6.2.1.1 mtCODH/ACS
-
Moorella thermoacetica

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.4 Ni-Fe-S center uses a Ni-Fe-S center called the C-cluster to reduce carbon dioxide to carbon monoxide and uses a second Ni-Fe-S center, called the A-cluster, to assemble acetyl-CoA from a methyl group, coenzyme A, and C-cluster-generated CO Moorella thermoacetica