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Literature summary extracted from

  • Hassan-Abdallah, A.; Zhao, G.; Chen, Z.W.; Mathews, F.S.; Schuman Jorns, M.
    Arginine 49 is a bifunctional residue important in catalysis and biosynthesis of monomeric sarcosine oxidase: a context-sensitive model for the electrostatic impact of arginine to lysine mutations (2008), Biochemistry, 47, 2913-2922.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.1 native and mutant enzyme Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.3.1 R49K mutant after reconstitution with FAD, sitting drop vapor diffusion method, high salt or high PEG conditions Bacillus sp. (in: Bacteria)

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.1 R49K 40fold decrease of turnover, 150fold decrease of catalytic efficiency, structure very similar to wild type enzyme Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.1 methylthioacetate
-
Bacillus sp. (in: Bacteria)
1.5.3.1 N-(cyclopropyl)glycin suicide substrate Bacillus sp. (in: Bacteria)
1.5.3.1 pyrrole-2-carboxylate
-
Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.1 0.01
-
sarcosine R49K mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
1.5.3.1 0.29
-
sarcosine native enzyme, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
1.5.3.1 7.6
-
sarcosine native enzyme, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
1.5.3.1 28.8
-
sarcosine R49K mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.1 sarcosine + O2 + H2O Bacillus sp. (in: Bacteria)
-
formaldehyde + glycine + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Bacillus sp. (in: Bacteria) P40859
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.1 recombinant native and mutant enzyme Bacillus sp. (in: Bacteria)

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.3.1 sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 mechanism suggested Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 additional information also oxidizes other amino acids containing a secondary amino group e.g. L-proline, and N-methyl-L-alanine Bacillus sp. (in: Bacteria) ?
-
?
1.5.3.1 sarcosine + O2 + H2O
-
Bacillus sp. (in: Bacteria) formaldehyde + glycine + H2O2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.1 monomeric sarcosine oxidase
-
Bacillus sp. (in: Bacteria)
1.5.3.1 MSOX
-
Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.1 2.7
-
sarcosine R49K mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
1.5.3.1 101
-
sarcosine native enzyme, pH 8.0, 25°C Bacillus sp. (in: Bacteria)

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.1 FAD covalently bound Bacillus sp. (in: Bacteria)