EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.3.1 | native and mutant enzyme | Bacillus sp. (in: Bacteria) |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.5.3.1 | R49K mutant after reconstitution with FAD, sitting drop vapor diffusion method, high salt or high PEG conditions | Bacillus sp. (in: Bacteria) |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.3.1 | R49K | 40fold decrease of turnover, 150fold decrease of catalytic efficiency, structure very similar to wild type enzyme | Bacillus sp. (in: Bacteria) |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.3.1 | methylthioacetate | - |
Bacillus sp. (in: Bacteria) | |
1.5.3.1 | N-(cyclopropyl)glycin | suicide substrate | Bacillus sp. (in: Bacteria) | |
1.5.3.1 | pyrrole-2-carboxylate | - |
Bacillus sp. (in: Bacteria) |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.3.1 | 0.01 | - |
sarcosine | R49K mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
1.5.3.1 | 0.29 | - |
sarcosine | native enzyme, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
1.5.3.1 | 7.6 | - |
sarcosine | native enzyme, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
1.5.3.1 | 28.8 | - |
sarcosine | R49K mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.3.1 | sarcosine + O2 + H2O | Bacillus sp. (in: Bacteria) | - |
formaldehyde + glycine + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.3.1 | Bacillus sp. (in: Bacteria) | P40859 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.3.1 | recombinant native and mutant enzyme | Bacillus sp. (in: Bacteria) |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 | mechanism suggested | Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.3.1 | additional information | also oxidizes other amino acids containing a secondary amino group e.g. L-proline, and N-methyl-L-alanine | Bacillus sp. (in: Bacteria) | ? | - |
? | |
1.5.3.1 | sarcosine + O2 + H2O | - |
Bacillus sp. (in: Bacteria) | formaldehyde + glycine + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.3.1 | monomeric sarcosine oxidase | - |
Bacillus sp. (in: Bacteria) |
1.5.3.1 | MSOX | - |
Bacillus sp. (in: Bacteria) |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.3.1 | 2.7 | - |
sarcosine | R49K mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
1.5.3.1 | 101 | - |
sarcosine | native enzyme, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.3.1 | FAD | covalently bound | Bacillus sp. (in: Bacteria) |