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Literature summary extracted from

  • Ruiz-Duenas, F.J.; Morales, M.; Mate, M.J.; Romero, A.; Martinez, M.J.; Smith, A.T.; Martinez, A.T.
    Site-directed mutagenesis of the catalytic tryptophan environment in Pleurotus eryngii versatile peroxidase (2008), Biochemistry, 47, 1685-1695.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.16 expression in Escherichia coli Pleurotus eryngii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.16 wild-type and mutant M247F Pleurotus eryngii

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.16 A260F kinetics similar to wild-type Pleurotus eryngii
1.11.1.16 K264A kinetics similar to wild-type Pleurotus eryngii
1.11.1.16 M247F 92% decrease in efficiency for oxidizing Reactive Black 5 Pleurotus eryngii
1.11.1.16 M247L kinetics similar to wild-type Pleurotus eryngii
1.11.1.16 N256D/R257D/A260F unstable, complete loss of activity Pleurotus eryngii
1.11.1.16 R257A/A260F 43% decrease in efficiency for oxidizing veratryl alcohol Pleurotus eryngii
1.11.1.16 R257D 83% decrease in efficiency for oxidizing veratryl alcohol Pleurotus eryngii
1.11.1.16 R257K 65% decrease in efficiency for oxidizing veratryl alcohol Pleurotus eryngii
1.11.1.16 R257L 3-fold increase in Km value for veratryl alcohol Pleurotus eryngii
1.11.1.16 S158D kinetics similar to wild-type Pleurotus eryngii
1.11.1.16 S158E kinetics similar to wild-type Pleurotus eryngii
1.11.1.16 S158E/R257D unstable, complete loss of activity Pleurotus eryngii
1.11.1.16 W164S complete loss of activity Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.16 0.0013
-
 Reactive Black 5 pH 3.5, 25°C, mutant M247L Pleurotus eryngii
1.11.1.16 0.0014
-
 Reactive Black 5 pH 3.5, 25°C, mutant S158D Pleurotus eryngii
1.11.1.16 0.0017
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant R257L Pleurotus eryngii
1.11.1.16 0.0019
-
 Reactive Black 5 pH 3.5, 25°C, mutant A260F Pleurotus eryngii
1.11.1.16 0.0022
-
 Reactive Black 5 pH 3.5, 25°C, mutant S158E Pleurotus eryngii
1.11.1.16 0.0025
-
 Reactive Black 5 pH 3.5, 25°C, mutant K264A Pleurotus eryngii
1.11.1.16 0.0026
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant M247L Pleurotus eryngii
1.11.1.16 0.0026
-
 Reactive Black 5 pH 3.5, 25°C, mutant R257K Pleurotus eryngii
1.11.1.16 0.0027
-
 Reactive Black 5 pH 3.5, 25°C, mutant R257L Pleurotus eryngii
1.11.1.16 0.0028
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant S158E Pleurotus eryngii
1.11.1.16 0.003
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, wild-type Pleurotus eryngii
1.11.1.16 0.0031
-
 Reactive Black 5 pH 3.5, 25°C, mutant M247F Pleurotus eryngii
1.11.1.16 0.0032
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant R257A/A260F Pleurotus eryngii
1.11.1.16 0.0034
-
 Reactive Black 5 pH 3.5, 25°C, wild-type Pleurotus eryngii
1.11.1.16 0.0035
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant M247F Pleurotus eryngii
1.11.1.16 0.0036
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant R257K Pleurotus eryngii
1.11.1.16 0.004
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant S158D Pleurotus eryngii
1.11.1.16 0.0049
-
 Reactive Black 5 pH 3.5, 25°C, mutant R257A/A260F Pleurotus eryngii
1.11.1.16 0.0065
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant K264A Pleurotus eryngii
1.11.1.16 0.009
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant A260F Pleurotus eryngii
1.11.1.16 1.65
-
 veratryl alcohol pH 3.0, 25°C, mutant S158E Pleurotus eryngii
1.11.1.16 4.11
-
 veratryl alcohol pH 3.0, 25°C, mutant M247F Pleurotus eryngii
1.11.1.16 4.13
-
 veratryl alcohol pH 3.0, 25°C, wild-type Pleurotus eryngii
1.11.1.16 5.11
-
 veratryl alcohol pH 3.0, 25°C, mutant A260F Pleurotus eryngii
1.11.1.16 5.68
-
 veratryl alcohol pH 3.0, 25°C, mutant K264A Pleurotus eryngii
1.11.1.16 5.96
-
 veratryl alcohol pH 3.0, 25°C, mutant S158D Pleurotus eryngii
1.11.1.16 6.99
-
 veratryl alcohol pH 3.0, 25°C, mutant M247L Pleurotus eryngii
1.11.1.16 13.5
-
 veratryl alcohol pH 3.0, 25°C, mutant R257A/A260F Pleurotus eryngii
1.11.1.16 13.5
-
 veratryl alcohol pH 3.0, 25°C, mutant R257L Pleurotus eryngii
1.11.1.16 14.2
-
 veratryl alcohol pH 3.0, 25°C, mutant R257K Pleurotus eryngii
1.11.1.16 54.7
-
 veratryl alcohol pH 3.0, 25°C, mutant R257D Pleurotus eryngii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.16 Manganese Km value Mn2+ is 0.181 mM for wild-type Pleurotus eryngii

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.16 Pleurotus eryngii O94753
-
-

Storage Stability

EC Number Storage Stability Organism
1.11.1.16 4°C, pH 4-7, wild-type is stable for 96 h Pleurotus eryngii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.16 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pleurotus eryngii ? + H2O
-
 ?
1.11.1.16 Reactive Black 5 + H2O2
-
 Pleurotus eryngii oxidized Reactive Black 5 + H2O
-
 ?
1.11.1.16 veratryl alcohol + H2O2 + H+
-
 Pleurotus eryngii veratraldehyde + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.16 0.4
-
 Reactive Black 5 pH 3.5, 25°C, mutant M247F Pleurotus eryngii
1.11.1.16 0.7
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant M247F Pleurotus eryngii
1.11.1.16 2.3
-
 Reactive Black 5 pH 3.5, 25°C, mutant R257K Pleurotus eryngii
1.11.1.16 3.1
-
 Reactive Black 5 pH 3.5, 25°C, mutant A260F Pleurotus eryngii
1.11.1.16 3.3
-
 Reactive Black 5 pH 3.5, 25°C, mutant M247L Pleurotus eryngii
1.11.1.16 4.2
-
 Reactive Black 5 pH 3.5, 25°C, mutant K264A Pleurotus eryngii
1.11.1.16 4.3
-
 veratryl alcohol pH 3.0, 25°C, mutant M247F Pleurotus eryngii
1.11.1.16 4.6
-
 Reactive Black 5 pH 3.5, 25°C, mutant S158E Pleurotus eryngii
1.11.1.16 4.9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant M247L Pleurotus eryngii
1.11.1.16 4.9
-
 Reactive Black 5 pH 3.5, 25°C, mutant S158D Pleurotus eryngii
1.11.1.16 5
-
 veratryl alcohol pH 3.0, 25°C, mutant M247L Pleurotus eryngii
1.11.1.16 5.5
-
 Reactive Black 5 pH 3.5, 25°C, wild-type Pleurotus eryngii
1.11.1.16 5.9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant A260F Pleurotus eryngii
1.11.1.16 7.5
-
 veratryl alcohol pH 3.0, 25°C, mutant A260F Pleurotus eryngii
1.11.1.16 7.7
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant S158D Pleurotus eryngii
1.11.1.16 8.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, wild-type Pleurotus eryngii
1.11.1.16 8.2
-
 veratryl alcohol pH 3.0, 25°C, mutant K264A Pleurotus eryngii
1.11.1.16 8.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant K264A Pleurotus eryngii
1.11.1.16 9.1
-
 Reactive Black 5 pH 3.5, 25°C, mutant R257A/A260F Pleurotus eryngii
1.11.1.16 9.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant S158E Pleurotus eryngii
1.11.1.16 9.5
-
 veratryl alcohol pH 3.0, 25°C, wild-type Pleurotus eryngii
1.11.1.16 9.6
-
 veratryl alcohol pH 3.0, 25°C, mutant S158D Pleurotus eryngii
1.11.1.16 9.6
-
 veratryl alcohol pH 3.0, 25°C, mutant S158E Pleurotus eryngii
1.11.1.16 10.9
-
 veratryl alcohol pH 3.0, 25°C, mutant R257K Pleurotus eryngii
1.11.1.16 11.6
-
 Reactive Black 5 pH 3.5, 25°C, mutant R257L Pleurotus eryngii
1.11.1.16 11.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant R257K Pleurotus eryngii
1.11.1.16 17.9
-
 veratryl alcohol pH 3.0, 25°C, mutant R257A/A260F Pleurotus eryngii
1.11.1.16 18.6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant R257A/A260F Pleurotus eryngii
1.11.1.16 19.6
-
 veratryl alcohol pH 3.0, 25°C, mutant R257D Pleurotus eryngii
1.11.1.16 22.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C, mutant R257L Pleurotus eryngii
1.11.1.16 27.3
-
 veratryl alcohol pH 3.0, 25°C, mutant R257L Pleurotus eryngii