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Literature summary extracted from

  • Han, Q.; Gao, Y.G.; Robinson, H.; Li, J.
    Structural insight into the mechanism of substrate specificity of Aedes kynurenine aminotransferase (2008), Biochemistry, 47, 1622-1630.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.13 expressed in a baculovirus/insect cell protein expression system Aedes aegypti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.7 Comparison of the active site residues of the Aedes aegypti enzyme-cysteine structure with those of the human isoform KAT I-phenylalanine structure reveals that Tyr286 in the Aedes aegypti enzyme is changed to Phe278 in the human enzyme, which may explain why the Aedes aegypti enzyme transaminates hydrophilic amino acids more efficiently than the human enzyme does Homo sapiens
2.6.1.7 in complex with its best amino acid substrates, glutamine and cysteine. Glutamine is found in both subunits of the biological dimer, and cysteine is found in one of the two subunits. Both substrates form external aldemines with pyridoxal 5'-phosphate in the structures. All the units with substrate are in the closed conformation form, and the unit without substrate is in the open form. Tyr286 in the Aedes aegypti enzyme is changed to Phe278 in the human enzyme, which may explain why the Aedes enzyme transaminates hydrophilic amino acids more efficiently than the human enzyme does Aedes aegypti
4.4.1.13 hanging drop vapour diffusion method, with 2 M ammonium sulfate and 0.1 M Tris at pH 8.5 Aedes aegypti

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Aedes aegypti
-
-
-
2.6.1.7 Homo sapiens
-
-
-
4.4.1.13 Aedes aegypti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Aedes aegypti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 L-cysteine + 2-oxobutanoate
-
 Aedes aegypti 2-aminobutanoate + 3-mercapto-2-oxopropanoate
-
 ?
2.6.1.7 L-glutamine + 2-oxobutanoate
-
 Aedes aegypti 2-oxoglutarate + 2-aminobutanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 homodimer x-ray crystallography Aedes aegypti

Synonyms

EC Number Synonyms Comment Organism
4.4.1.13 glutamine transaminase K
-
 Aedes aegypti
4.4.1.13 GTK
-
 Aedes aegypti
4.4.1.13 KAT also is a cysteine S-conjugate beta-lyase Aedes aegypti
4.4.1.13 kynurenine aminotransferase the enzyme is a multifunctional aminotransferase and also a cysteine S-conjugate beta-lyase Aedes aegypti

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.7 pyridoxal 5'-phosphate
-
 Aedes aegypti
4.4.1.13 pyridoxal 5'-phosphate
-
 Aedes aegypti