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Literature summary extracted from

  • Webb, L.J.; Boxer, S.G.
    Electrostatic fields near the active site of human aldose reductase: 1. New inhibitors and vibrational stark effect measurements (2008), Biochemistry, 47, 1588-1598.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.21 F115Y stable and active Homo sapiens
1.1.1.21 T113A stable and active Homo sapiens
1.1.1.21 T113A/F115Y 10fold decrease in kcat value, does not bind to inhibitor (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid Homo sapiens
1.1.1.21 T113V inhibition constants and vibrational frequencies of inhibitor (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid in the active site Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.21 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Homo sapiens
-
 isoform hALR2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 DL-glyceraldehyde + NADPH
-
 Homo sapiens glycerol + NADP+
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.21 0.00004
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant T113A/C303D, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.00006
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant T113A/C303NpH 7.0, 22°C Homo sapiens
1.1.1.21 0.00011
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant T113A, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.00029
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant S302R/C303D, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.00029
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant T113V, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.00051
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant C303D, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.00062
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant F115Y, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.0008
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant C303N, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.002
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant C80N, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.002
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid wild-type, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.0039
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant T113Y, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.004
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant T113Y/S302R/C303D, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.0046
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant C80A, pH 7.0, 22°C Homo sapiens
1.1.1.21 0.0078
-
 (5-chloro-2-[(4-cyanobenzyl)carbamoyl]phenoxy)acetic acid mutant C80N/T113Y/S302R/C303D, pH 7.0, 22°C Homo sapiens