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Literature summary extracted from

  • Yang, X.; Dubnau, E.; Smith, I.; Sampson, N.S.
    Rv1106c from Mycobacterium tuberculosis is a 3beta-hydroxysteroid dehydrogenase (2007), Biochemistry, 46, 9058-9067.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.145 expression in Mycobacterium smegmatis Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.145 additional information disruption of gene Rv1106c abrogates bacterial cholesterol oxidation activity Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.145 NADH mixed type inhibition Mycobacterium tuberculosis
1.1.1.145 trilostane competitive Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.023
-
 pregnenolone pH 8.5, 37°C Mycobacterium tuberculosis
1.1.1.145 0.172
-
 NAD+ cosubstrate pregnenolone, pH 8.5, 37°C Mycobacterium tuberculosis
1.1.1.145 0.221
-
 dehydroepiandrosterone pH 8.5, 37°C Mycobacterium tuberculosis
1.1.1.145 0.27
-
 NAD+ cosubstrate dehydroepiandrosterone, pH 8.5, 37°C Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.145 intracellular
-
 Mycobacterium tuberculosis 5622
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Mycobacterium tuberculosis
-
 product of Rv1106c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.145 recombinant protein Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.145 a 3beta-hydroxy-DELTA5-steroid + NAD+ = a 3-oxo-DELTA5-steroid + NADH + H+ enzyme forms a ternary complex with NAD+ binding before sterol binding Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 cholesterol + NAD+
-
 Mycobacterium tuberculosis cholest-5-en-3-one + NADH
-
 ?
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Mycobacterium tuberculosis androst-5-en-3,17-dione + NADH + H+
-
 ?
1.1.1.145 pregnenolone + NAD+
-
 Mycobacterium tuberculosis progesterone + NADH + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.145 1.48
-
 pregnenolone pH 8.5, 37°C Mycobacterium tuberculosis
1.1.1.145 11.6
-
 dehydroepiandrosterone pH 8.5, 37°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.145 8.5 9.5
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.145 0.197
-
 trilostane pH 8.5, 37°C Mycobacterium tuberculosis
1.1.1.145 0.245
-
 NADH pH 8.5, 37°C Mycobacterium tuberculosis