Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Szaleniec, M.; Hagel, C.; Menke, M.; Nowak, P.; Witko, M.; Heider, J.
    Kinetics and mechanism of oxygen-independent hydrocarbon hydroxylation by ethylbenzene dehydrogenase (2007), Biochemistry, 46, 7637-7646.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.99.2 O2 the enzyme is quickly inactivated in air in its reduced state Azoarcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.99.2 additional information
-
 additional information kinetics Azoarcus sp.
1.17.99.2 0.0018
-
 3-ethylpyridine pH 7.5, 30°C Azoarcus sp.
1.17.99.2 0.0023
-
 2-ethylpyrrole pH 7.5, 30°C Azoarcus sp.
1.17.99.2 0.0052
-
 2-Ethylphenol pH 7.5, 30°C Azoarcus sp.
1.17.99.2 0.0093
-
 3-Ethylphenol pH 7.5, 30°C Azoarcus sp.
1.17.99.2 0.0243
-
 2-ethylaniline pH 7.5, 30°C Azoarcus sp.
1.17.99.2 0.0247
-
 2-ethylfuran pH 7.5, 30°C Azoarcus sp.
1.17.99.2 0.0283
-
 ferricenium pH 7.5, 30°C Azoarcus sp.
1.17.99.2 0.0405
-
 4-Ethylphenol pH 7.5, 30°C Azoarcus sp.
1.17.99.2 0.045
-
 ethylbenzene pH 7.5, 30°C Azoarcus sp.
1.17.99.2 0.0665
-
 2-ethylthiophene pH 7.5, 30°C Azoarcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.99.2 Fe2+ a molybdenum/iron-sulfur/heme protein Azoarcus sp.
1.17.99.2 Molybdenum a molybdenum/iron-sulfur/heme protein Azoarcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.99.2 155000
-
-
 Azoarcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.99.2 ethylbenzene + H2O + acceptor Azoarcus sp.
-
 (S)-1-phenylethanol + reduced acceptor
-
 ?
1.17.99.2 ethylbenzene + H2O + acceptor Azoarcus sp. EbN1
-
 (S)-1-phenylethanol + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.99.2 Azoarcus sp.
-
 i.e. Aromatoleum aromaticum
-
1.17.99.2 Azoarcus sp. EbN1
-
 i.e. Aromatoleum aromaticum
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.17.99.2 the enzyme is quickly inactivated in air in its reduced state Azoarcus sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.99.2 ethylbenzene + H2O + acceptor = (S)-1-phenylethanol + reduced acceptor kinetics and mechanism of oxygen-independent hydrocarbon hydroxylation, quantitative structure-activity relationship modeling Azoarcus sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.99.2 1,4-diethylbenzene + H2O + ferricenium
-
 Azoarcus sp. 1-(4-ethylphenyl)ethanol + 1,1'-(1,4-phenylene)diethanol + ferrocenium
-
 ?
1.17.99.2 1,4-diethylbenzene + H2O + ferricenium
-
 Azoarcus sp. EbN1 1-(4-ethylphenyl)ethanol + 1,1'-(1,4-phenylene)diethanol + ferrocenium
-
 ?
1.17.99.2 2-ethylaniline + H2O + ferricenium
-
 Azoarcus sp. ?
-
 ?
1.17.99.2 2-ethylaniline + H2O + ferricenium
-
 Azoarcus sp. EbN1 ?
-
 ?
1.17.99.2 2-ethylfuran + H2O + ferricenium
-
 Azoarcus sp. 1-(2-furyl)ethanol + ferrocenium
-
 ?
1.17.99.2 2-ethylfuran + H2O + ferricenium
-
 Azoarcus sp. ?
-
 ?
1.17.99.2 2-ethylphenol + H2O + ferricenium
-
 Azoarcus sp. 2-(1-hydroxyethyl)phenol + ferrocenium
-
 ?
1.17.99.2 2-ethylpyrrole + H2O + ferricenium
-
 Azoarcus sp. ?
-
 ?
1.17.99.2 2-ethylthiophene + H2O + ferricenium
-
 Azoarcus sp. 1-thien-2-ylethanol + ferrocenium
-
 ?
1.17.99.2 2-ethylthiophene + H2O + ferricenium
-
 Azoarcus sp. ?
-
 ?
1.17.99.2 3-ethylphenol + H2O + ferricenium
-
 Azoarcus sp. 3-(1-hydroxyethyl)phenol + ferrocenium
-
 ?
1.17.99.2 3-ethylpyridine + H2O + ferricenium
-
 Azoarcus sp. ?
-
 ?
1.17.99.2 4-ethylanisole + H2O + ferricenium
-
 Azoarcus sp. 1-(4-methoxyphenyl)ethanol + ferrocenium
-
 ?
1.17.99.2 4-ethylbiphenyl + H2O + ferricenium
-
 Azoarcus sp. 1-(1,1'-biphenyl-4-yl)ethanol + reduced ferrocenium
-
 ?
1.17.99.2 4-ethylbiphenyl + H2O + ferricenium
-
 Azoarcus sp. EbN1 1-(1,1'-biphenyl-4-yl)ethanol + reduced ferrocenium
-
 ?
1.17.99.2 4-ethylphenol + H2O + ferricenium best substrate Azoarcus sp. 4-(1-hydroxyethyl)phenol + ferrocenium
-
 ?
1.17.99.2 4-ethyltoluene + H2O + ferricenium
-
 Azoarcus sp. 1-(4-methylphenyl)ethanol + ferrocenium
-
 ?
1.17.99.2 4-propylphenol + H2O + ferricenium
-
 Azoarcus sp. 4-(1-hydroxypropyl)phenol + ferrocenium
-
 ?
1.17.99.2 ethylbenzene + H2O + acceptor
-
 Azoarcus sp. (S)-1-phenylethanol + reduced acceptor
-
 ?
1.17.99.2 ethylbenzene + H2O + acceptor
-
 Azoarcus sp. EbN1 (S)-1-phenylethanol + reduced acceptor
-
 ?
1.17.99.2 ethylbenzene + H2O + ferricenium EBDH catalyzes the oxygen-independent, stereospecific hydroxylation of ethylbenzene to (S)-1-phenylethanol, the direct anaerobic oxidation of a nonactivated hydrocarbon Azoarcus sp. (S)-1-phenylethanol + ferrocenium
-
 ?
1.17.99.2 ethylidenecyclohexane + H2O + ferricenium
-
 Azoarcus sp. ?
-
 ?
1.17.99.2 additional information substrate specificity, product identification by GC- and LC-MS, overview Azoarcus sp. ?
-
 ?
1.17.99.2 additional information substrate specificity, product identification by GC- and LC-MS, overview Azoarcus sp. EbN1 ?
-
 ?
1.17.99.2 propylbenzene + H2O + ferricenium
-
 Azoarcus sp. (S)-1-phenylpropanol + ferrocenium
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.99.2 More quantitative structure-activity relationship modeling Azoarcus sp.
1.17.99.2 trimer
-
 Azoarcus sp.

Synonyms

EC Number Synonyms Comment Organism
1.17.99.2 EBDH
-
 Azoarcus sp.
1.17.99.2 ethylbenzene dehydrogenase
-
 Azoarcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.99.2 30
-
 assay at Azoarcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.99.2 additional information
-
 additional information relative kcat values Azoarcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.99.2 7.5
-
 assay at Azoarcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.99.2 heme a molybdenum/iron-sulfur/heme protein Azoarcus sp.