BRENDA - Enzyme Database

Mechanistic and structural analysis of human spermidine/spermine N1-acetyltransferase

Hegde, S.S.; Chandler, J.; Vetting, M.W.; Yu, M.; Blanchard, J.S.; Biochemistry 46, 7187-7195 (2007) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.3.1.57
expressed in Escherichia coli, strains Nova Blue and BL21(DE3), plasmid pET-28a
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
2.3.1.57
three-dimensional crystal structure of SSAT with bound bisubstrate inhibitor, solved at 2.3 A resolution, hanging drop method, data and refinement statistics
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.57
N1-spermine-acetyl-CoA
bisubstrate analogue, bisubstrate inhibition patterns determined, linear, competitive inhibition against sperimidine and acetyl-CoA determined
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.57
additional information
-
additional information
no detectable activity for putrescine and cadaverine
Homo sapiens
2.3.1.57
0.0038
-
acetyl-CoA
measured at fixed saturating concentration of spermidine
Homo sapiens
2.3.1.57
0.0057
-
spermine
measured at fixed saturating concentrations of acetyl-CoA
Homo sapiens
2.3.1.57
0.022
-
spermidine
measured at fixed saturating concentrations of acetyl-CoA
Homo sapiens
2.3.1.57
0.107
-
1,3-diaminopropane
measured at fixed saturating concentrations of acetyl-CoA
Homo sapiens
2.3.1.57
0.194
-
diethylenetriamine
measured at fixed saturating concentrations of acetyl-CoA
Homo sapiens
2.3.1.57
0.196
-
ethylenediamine
measured at fixed saturating concentrations of acetyl-CoA
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.57
acetyl-CoA + spermidine
Homo sapiens
-
CoA + N1-acetylspermidine
-
-
?
2.3.1.57
acetyl-CoA + spermine
Homo sapiens
N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells
CoA + N1-acetylspermine
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.57
Homo sapiens
P21673
hSSAT gene
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.57
gel filtration, recombinant protein, all purification procedures at 4°C
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.57
commercial preparation
cDNA clone of human SSAT
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.57
additional information
-
kinetic and mechanistic characterization, determination of bisubstrate inhibition patterns, three-dimensional structure of the recombinant human SSAT protein with bound bisubstrate inhibitor, acid/base assisted reaction catalyzed by SSAT involves a ternary complex, proposed on the base of kinetic properties, pH dependence and structure analysis
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.57
acetyl-CoA + 1,3-diaminopropane
-
685168
Homo sapiens
CoA + N1-acetyl-1,3-diaminopropane
-
-
-
?
2.3.1.57
acetyl-CoA + diethylenetriamine
-
685168
Homo sapiens
CoA + N1-acetyl-diethylenetriamine
-
-
-
?
2.3.1.57
acetyl-CoA + ethylenediamine
-
685168
Homo sapiens
CoA + N1-acetyl-ethylenediamine
-
-
-
?
2.3.1.57
acetyl-CoA + spermidine
-
685168
Homo sapiens
CoA + N1-acetylspermidine
-
-
-
?
2.3.1.57
acetyl-CoA + spermine
N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells
685168
Homo sapiens
CoA + N1-acetylspermine
-
-
-
?
2.3.1.57
acetyl-CoA + spermine
N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells, chemical and kinetic mechanism for acetyl transfer activity by recombinant human SSAT protein proposed
685168
Homo sapiens
CoA + N1-acetylspermine
-
-
-
?
2.3.1.57
chloroacetyl-CoA + spermine
N1-chloroacetylation of spermine performed by hSSAT protein, pH 7.5 and 2 microM recombinant human SSAT protein at room temperature for one hour, identity of the product confirmed by mass spectrometry
685168
Homo sapiens
N1-spermine-acetyl-CoA + ?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.3.1.57
SSAT
-
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.57
8
-
determined by pH activity profile
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.57
6.7
9.1
activity monitored every 0.3 pH units, bell shaped pH activity profile, depending on ionization state of two groups with apparent pKa values of 7.27 and 8.87
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.57
0.006
-
N1-spermine-acetyl-CoA
bisubstrate analogue, linear, competitive inhibition, structure of hSSAT with bound N1-spermine-acetyl-CoA determined
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.57
expressed in Escherichia coli, strains Nova Blue and BL21(DE3), plasmid pET-28a
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.3.1.57
three-dimensional crystal structure of SSAT with bound bisubstrate inhibitor, solved at 2.3 A resolution, hanging drop method, data and refinement statistics
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.57
N1-spermine-acetyl-CoA
bisubstrate analogue, bisubstrate inhibition patterns determined, linear, competitive inhibition against sperimidine and acetyl-CoA determined
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.57
0.006
-
N1-spermine-acetyl-CoA
bisubstrate analogue, linear, competitive inhibition, structure of hSSAT with bound N1-spermine-acetyl-CoA determined
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.57
additional information
-
additional information
no detectable activity for putrescine and cadaverine
Homo sapiens
2.3.1.57
0.0038
-
acetyl-CoA
measured at fixed saturating concentration of spermidine
Homo sapiens
2.3.1.57
0.0057
-
spermine
measured at fixed saturating concentrations of acetyl-CoA
Homo sapiens
2.3.1.57
0.022
-
spermidine
measured at fixed saturating concentrations of acetyl-CoA
Homo sapiens
2.3.1.57
0.107
-
1,3-diaminopropane
measured at fixed saturating concentrations of acetyl-CoA
Homo sapiens
2.3.1.57
0.194
-
diethylenetriamine
measured at fixed saturating concentrations of acetyl-CoA
Homo sapiens
2.3.1.57
0.196
-
ethylenediamine
measured at fixed saturating concentrations of acetyl-CoA
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.57
acetyl-CoA + spermidine
Homo sapiens
-
CoA + N1-acetylspermidine
-
-
?
2.3.1.57
acetyl-CoA + spermine
Homo sapiens
N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells
CoA + N1-acetylspermine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.57
gel filtration, recombinant protein, all purification procedures at 4°C
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.57
commercial preparation
cDNA clone of human SSAT
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.57
additional information
-
kinetic and mechanistic characterization, determination of bisubstrate inhibition patterns, three-dimensional structure of the recombinant human SSAT protein with bound bisubstrate inhibitor, acid/base assisted reaction catalyzed by SSAT involves a ternary complex, proposed on the base of kinetic properties, pH dependence and structure analysis
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.57
acetyl-CoA + 1,3-diaminopropane
-
685168
Homo sapiens
CoA + N1-acetyl-1,3-diaminopropane
-
-
-
?
2.3.1.57
acetyl-CoA + diethylenetriamine
-
685168
Homo sapiens
CoA + N1-acetyl-diethylenetriamine
-
-
-
?
2.3.1.57
acetyl-CoA + ethylenediamine
-
685168
Homo sapiens
CoA + N1-acetyl-ethylenediamine
-
-
-
?
2.3.1.57
acetyl-CoA + spermidine
-
685168
Homo sapiens
CoA + N1-acetylspermidine
-
-
-
?
2.3.1.57
acetyl-CoA + spermine
N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells
685168
Homo sapiens
CoA + N1-acetylspermine
-
-
-
?
2.3.1.57
acetyl-CoA + spermine
N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells, chemical and kinetic mechanism for acetyl transfer activity by recombinant human SSAT protein proposed
685168
Homo sapiens
CoA + N1-acetylspermine
-
-
-
?
2.3.1.57
chloroacetyl-CoA + spermine
N1-chloroacetylation of spermine performed by hSSAT protein, pH 7.5 and 2 microM recombinant human SSAT protein at room temperature for one hour, identity of the product confirmed by mass spectrometry
685168
Homo sapiens
N1-spermine-acetyl-CoA + ?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.57
8
-
determined by pH activity profile
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.57
6.7
9.1
activity monitored every 0.3 pH units, bell shaped pH activity profile, depending on ionization state of two groups with apparent pKa values of 7.27 and 8.87
Homo sapiens