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Literature summary extracted from

  • Magnusson, O.T.; RoseFigura, J.M.; Toyama, H.; Schwarzenbacher, R.; Klinman, J.P.
    Pyrroloquinoline quinone biogenesis: characterization of PqqC and its H84N and H84A active site variants (2007), Biochemistry, 46, 7174-7186.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.11 expressed in Escherichia coli BL21(DE3) cells Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.11 H84A active site mutant with reduced activity Klebsiella pneumoniae
1.3.3.11 H84N active site mutant with reduced activity Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.11 28000
-
 SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.11 additional information Klebsiella pneumoniae catalyzes the final step of PQQ formation, which involves a ring closure and an overall eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid in the absence of a redox-active metal or cofactor ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.11 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.11 Q-Sepharose column chromatography and Ultrogel AcA54 gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.11 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2
-
 Klebsiella pneumoniae 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
1.3.3.11 additional information catalyzes the final step of PQQ formation, which involves a ring closure and an overall eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid in the absence of a redox-active metal or cofactor Klebsiella pneumoniae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.11 PqqC
-
 Klebsiella pneumoniae