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Literature summary extracted from

  • Kona, F.; Xu, X.; Martin, P.; Kuzmic, P.; Gatti, D.L.
    Structural and mechanistic changes along an engineered path from metallo to nonmetallo 3-deoxy-D-manno-octulosonate 8-phosphate synthases (2007), Biochemistry, 46, 4532-4544.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.55 expressed in Escherichia coli Aquifex aeolicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.55
-
Aquifex aeolicus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.55 C11N mutant is not capable of binding metal and lacks the structural asymmetry among subunits with regard to substrate binding and conformation of the L7 loop, shows decreased thermal stability Aquifex aeolicus
2.5.1.55 C11N/S235P/Q237A mutant is not capable of binding metal and lacks the structural asymmetry among subunits with regard to substrate binding and conformation of the L7 loop, shows decreased thermal stability Aquifex aeolicus
2.5.1.55 P10M/C11N mutant is not capable of binding metal and lacks the structural asymmetry among subunits with regard to substrate binding and conformation of the L7 loop, shows decreased thermal stability Aquifex aeolicus
2.5.1.55 P10M/C11N/S235P/Q237A mutant is not capable of binding metal and lacks the structural asymmetry among subunits with regard to substrate binding and conformation of the L7 loop, shows decreased thermal stability Aquifex aeolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.55 Cd2+ 0.05 mM Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Aquifex aeolicus O66496
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55
-
Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
-
Aquifex aeolicus 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.55 3-deoxy-D-manno-octulosonate 8-phosphate synthase
-
Aquifex aeolicus
2.5.1.55 EC 4.1.2.16 formerly Aquifex aeolicus
2.5.1.55 Kdo8P synthase
-
Aquifex aeolicus
2.5.1.55 KDO8PS
-
Aquifex aeolicus