EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.6.1.8 | actin | - |
Mammalia |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.6.1.8 | for expression in insect amd mammalian cells | Mammalia |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.6.1.8 | Myo1b-GAGEGA | Myo1b in which wild-type loop 4 is replaced with loop 4 of Dictyostelium myosin II, GAGEGA | Mammalia |
5.6.1.8 | Myo1b-NGLD | Myo1b in which wild-type loop 4 is truncated to NGLD | Mammalia |
5.6.1.8 | Myo1b1IQ-GAGEGA | Myo1b1IQ in which wild-type loop 4 is replaced with loop 4 of Dictyostelium myosin II, GAGEGA | Mammalia |
5.6.1.8 | Myo1b1IQ-NGLD | Myo1b1IQ in which wild-type loop 4 is truncated to NGLD | Mammalia |
5.6.1.8 | Myo1b1IQ-WT | wild-type Myo1b truncated after the first IQ domain at amino acid 728 containing the wild-type loop 4 sequence, RMNGLDES | Mammalia |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.6.1.8 | tropomyosin | - |
Mammalia |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.6.1.8 | 0.008 | - |
ATP | Myo1b1IQ-GAGEGA, pCa 8.9 | Mammalia | |
5.6.1.8 | 0.009 | - |
ATP | Myo1b1IQ-GAGEGA, pCa 4.6 with tropomyosin-2 | Mammalia | |
5.6.1.8 | 0.009 | - |
ATP | Myo1b1IQ-NGLD, pCa 4.6 | Mammalia | |
5.6.1.8 | 0.009 | - |
ATP | Myo1b1IQ-WT, pCa 4.6 | Mammalia | |
5.6.1.8 | 0.009 | - |
ATP | Myo1b1IQ-WT, pCa 8.9 | Mammalia | |
5.6.1.8 | 0.01 | - |
ATP | Myo1b1IQ-GAGEGA, pCa 4.6 | Mammalia | |
5.6.1.8 | 0.011 | - |
ATP | Myo1b1IQ-NGLD, pCa 8.9 | Mammalia | |
5.6.1.8 | 0.011 | - |
ATP | Myo1b1IQ-WT, pCa 4.6 with tropomyosin-2 | Mammalia | |
5.6.1.8 | 0.013 | - |
ATP | Myo1b1IQ-NGLD, pCa 4.6 with tropomyosin-2 | Mammalia |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.6.1.8 | Mg2+ | - |
Mammalia |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.1.8 | ATP + H2O + myosin bound to actin filament at position n | Mammalia | - |
ADP + phosphate + myosin bound to actin filament at position n+1 | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.1.8 | Mammalia | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.1.8 | ATP + H2O + myosin bound to actin filament at position n | - |
Mammalia | ADP + phosphate + myosin bound to actin filament at position n+1 | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.6.1.8 | actin activated myosin ATPase | - |
Mammalia |
5.6.1.8 | actomyosin 1b ATPase | - |
Mammalia |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.6.1.8 | 37 | - |
activity assay | Mammalia |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.6.1.8 | 7.5 | - |
activity assay | Mammalia |