EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.16 | isoproterenol | stimulates the phosphorylation activity of GRK5 by10-15fold at 0.001 mM | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.16 | Calmodulin | inhibits the phosphorylation of rhodopsin by GRK5 | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.11.15 | membrane | - |
Homo sapiens | 16020 | - |
2.7.11.16 | membrane | - |
Homo sapiens | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.15 | Mg2+ | - |
Homo sapiens | |
2.7.11.16 | Mg2+ | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.15 | ATP + [beta2-adrenergic receptor] | Homo sapiens | - |
ADP + [beta2-adrenergic receptor] phosphate | - |
? | |
2.7.11.16 | ATP + [beta2-adrenergic receptor] | Homo sapiens | GRK5 plays a distinctive role in the phosphorylation of the beta2AR | ADP + [beta2-adrenergic receptor] phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.15 | Homo sapiens | - |
- |
- |
2.7.11.16 | Homo sapiens | P34947 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.11.16 | partial purification of GRK5 from HEK-293 cell membranes, separation from GRK2 and GRK6, overview | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.11.15 | HEK-293 cell | - |
Homo sapiens | - |
2.7.11.16 | HEK-293 cell | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.11.15 | additional information | - |
- |
Homo sapiens |
2.7.11.16 | additional information | - |
- |
Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.15 | ATP + rhodopsin | cf. EC 2.7.11.14, rhodopsin is also a substrate of GRK2 | Homo sapiens | ADP + rhodopsin phosphate | - |
? | |
2.7.11.15 | ATP + [beta2-adrenergic receptor] | - |
Homo sapiens | ADP + [beta2-adrenergic receptor] phosphate | - |
? | |
2.7.11.15 | ATP + [beta2-adrenergic receptor] | recombinant FLAG-tagged beta2-adrenergic receptor stably expressed in HEK-293 cells | Homo sapiens | ADP + [beta2-adrenergic receptor] phosphate | - |
? | |
2.7.11.16 | ATP + rhodopsin | cf. EC 2.7.11.14, rhodopsin is also a substrate of GRK5 | Homo sapiens | ADP + rhodopsin phosphate | - |
? | |
2.7.11.16 | ATP + [beta2-adrenergic receptor] | GRK5 plays a distinctive role in the phosphorylation of the beta2AR | Homo sapiens | ADP + [beta2-adrenergic receptor] phosphate | - |
? | |
2.7.11.16 | ATP + [beta2-adrenergic receptor] | cf. EC 2.7.11.15, recombinant FLAG-tagged beta2-adrenergic receptor stably expressed in HEK-293 cells | Homo sapiens | ADP + [beta2-adrenergic receptor] phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.11.15 | GRK2 | - |
Homo sapiens |
2.7.11.16 | GRK5 | - |
Homo sapiens |
2.7.11.16 | More | cf. EC 2.7.11.15 and EC 2.7.11.14 | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.15 | 30 | - |
assay at | Homo sapiens |
2.7.11.16 | 30 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.11.15 | 7 | - |
assay at | Homo sapiens |
2.7.11.16 | 7 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.15 | ATP | - |
Homo sapiens | |
2.7.11.16 | ATP | - |
Homo sapiens |