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Literature summary extracted from

  • Tran, T.M.; Jorgensen, R.; Clark, R.B.
    Phosphorylation of the beta2-adrenergic receptor in plasma membranes by intrinsic GRK5 (2007), Biochemistry, 46, 14438-14449.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.16 isoproterenol stimulates the phosphorylation activity of GRK5 by10-15fold at 0.001 mM Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.16 Calmodulin inhibits the phosphorylation of rhodopsin by GRK5 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.15 membrane
-
 Homo sapiens 16020
-
2.7.11.16 membrane
-
 Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.15 Mg2+
-
 Homo sapiens
2.7.11.16 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.15 ATP + [beta2-adrenergic receptor] Homo sapiens
-
 ADP + [beta2-adrenergic receptor] phosphate
-
 ?
2.7.11.16 ATP + [beta2-adrenergic receptor] Homo sapiens GRK5 plays a distinctive role in the phosphorylation of the beta2AR ADP + [beta2-adrenergic receptor] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Homo sapiens
-
-
-
2.7.11.16 Homo sapiens P34947
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.16 partial purification of GRK5 from HEK-293 cell membranes, separation from GRK2 and GRK6, overview Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.15 HEK-293 cell
-
 Homo sapiens
-
2.7.11.16 HEK-293 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.15 additional information
-
-
 Homo sapiens
2.7.11.16 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + rhodopsin cf. EC 2.7.11.14, rhodopsin is also a substrate of GRK2 Homo sapiens ADP + rhodopsin phosphate
-
 ?
2.7.11.15 ATP + [beta2-adrenergic receptor]
-
 Homo sapiens ADP + [beta2-adrenergic receptor] phosphate
-
 ?
2.7.11.15 ATP + [beta2-adrenergic receptor] recombinant FLAG-tagged beta2-adrenergic receptor stably expressed in HEK-293 cells Homo sapiens ADP + [beta2-adrenergic receptor] phosphate
-
 ?
2.7.11.16 ATP + rhodopsin cf. EC 2.7.11.14, rhodopsin is also a substrate of GRK5 Homo sapiens ADP + rhodopsin phosphate
-
 ?
2.7.11.16 ATP + [beta2-adrenergic receptor] GRK5 plays a distinctive role in the phosphorylation of the beta2AR Homo sapiens ADP + [beta2-adrenergic receptor] phosphate
-
 ?
2.7.11.16 ATP + [beta2-adrenergic receptor] cf. EC 2.7.11.15, recombinant FLAG-tagged beta2-adrenergic receptor stably expressed in HEK-293 cells Homo sapiens ADP + [beta2-adrenergic receptor] phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.15 GRK2
-
 Homo sapiens
2.7.11.16 GRK5
-
 Homo sapiens
2.7.11.16 More cf. EC 2.7.11.15 and EC 2.7.11.14 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at Homo sapiens
2.7.11.16 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.15 7
-
 assay at Homo sapiens
2.7.11.16 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Homo sapiens
2.7.11.16 ATP
-
 Homo sapiens