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Literature summary extracted from

  • Kontou, M.; Pournaras, S.; Kristo, I.; Ikonomidis, A.; Maniatis, A.N.; Stathopoulos, C.
    Molecular cloning and biochemical characterization of VIM-12, a novel hybrid VIM-1/VIM-2 metallo-beta-lactamase from a Klebsiella pneumoniae clinical isolate, reveal atypical substrate specificity (2007), Biochemistry, 46, 13170-13178.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli BL21 cells Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 ceftazidime acts as a very weak simple noncompetitive inhibitor Klebsiella pneumoniae
3.5.2.6 Imipenem
-
 Klebsiella pneumoniae
3.5.2.6 KCl concentrations of KCl higher than 20 mM weaken slightly the hydrolytic ability of VIM-12 Klebsiella pneumoniae
3.5.2.6 meropenem
-
 Klebsiella pneumoniae
3.5.2.6 additional information no inhibitory effect by aztreonam Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.094
-
 Imipenem
-
 Klebsiella pneumoniae
3.5.2.6 0.28
-
 penicillin G
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Zn2+ dependent Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Klebsiella pneumoniae
-
-
-
3.5.2.6 Klebsiella pneumoniae 2873
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 Ni-NTA column chromatography Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 imipenem + H2O moderate rate of hydrolysis Klebsiella pneumoniae (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O moderate rate of hydrolysis Klebsiella pneumoniae 2873 (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 additional information no hydrolysis of aztreonam, meropenem, ceftazidime, cefoxitin, and cefotaxime Klebsiella pneumoniae ?
-
 ?
3.5.2.6 additional information no hydrolysis of aztreonam, meropenem, ceftazidime, cefoxitin, and cefotaxime Klebsiella pneumoniae 2873 ?
-
 ?
3.5.2.6 penicillin G + H2O efficient hydrolysis Klebsiella pneumoniae (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 penicillin G + H2O efficient hydrolysis Klebsiella pneumoniae 2873 (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 MBL
-
 Klebsiella pneumoniae
3.5.2.6 metallo-beta-lactamase
-
 Klebsiella pneumoniae
3.5.2.6 VIM-12 a hybrid VIM-1/VIM-2 metallo-beta-lactamase Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.6 40
-
-
 Klebsiella pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.028
-
 Imipenem at 40?C in 50 mM HEPES buffer (pH 6.8) Klebsiella pneumoniae
3.5.2.6 34
-
 penicillin G at 40?C in 50 mM HEPES buffer (pH 6.8) Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.6 6.7
-
-
 Klebsiella pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.6 0.0042
-
 meropenem at 40?C in 50 mM HEPES buffer (pH 6.8) Klebsiella pneumoniae
3.5.2.6 0.0806
-
 Imipenem at 40?C in 50 mM HEPES buffer (pH 6.8) Klebsiella pneumoniae