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Literature summary extracted from

  • Boubacar, A.K.; Pethe, S.; Mahy, J.P.; Lederer, F.
    Flavocytochrome b2: reactivity of its flavin with molecular oxygen (2007), Biochemistry, 46, 13080-13088.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.3 recombinant wild-type Fcb2 and its recombinant FDH domain (FMN-binding domain) are expressed in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.3 additional information the separately engineered flavodehydrogenase domain produces superoxide anion in its slow reaction with oxygen. This reaction apparently also takes place in the holoenzyme when oxygen is the sole electron acceptor, because the heme domain autoxidation is also slow. This is not unexpected in view of the heme domain mobility relative to the tetrameric flavodehydrogenase core. Reaction is so slow that it cannot compete with the normal electron flow in the presence of monoelectronic acceptors, such as ferricyanide and cytochrome c Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Saccharomyces cerevisiae P00175
-
-

Storage Stability

EC Number Storage Stability Organism
1.1.2.3 -70°C, in 0.1 M phosphate buffer, 1 mM EDTA, pH 7, 10 mM DL-lactate is added to the Fcb2 preparations to keep the enzyme in the reduced state Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 L-lactate + ferricyanide
-
 Saccharomyces cerevisiae pyruvate + ferrocyanide + H+
-
 ?
1.1.2.3 L-lactate + O2 the FDH domain reacts slowly with oxygen with formation of superoxide anion when separated from its natural electron acceptor, whether isolated or included in the holoenzyme Saccharomyces cerevisiae ? + superoxide anion
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.3 Fcb2
-
 Saccharomyces cerevisiae
1.1.2.3 flavocytochrome b2
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 FMN
-
 Saccharomyces cerevisiae