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Literature summary extracted from

  • Taylor, E.A.; Rinaldo-Matthis, A.; Li, L.; Ghanem, M.; Hazleton, K.Z.; Cassera, M.B.; Almo, S.C.; Schramm, V.L.
    Anopheles gambiae purine nucleoside phosphorylase: catalysis, structure, and inhibition (2007), Biochemistry, 46, 12405-12415.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expression in Escherichia coli Anopheles gambiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.1 crystal structure of AgPNP is determined in complex with 5'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H and phosphate to a resolution of 2.2 A Anopheles gambiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.05
-
 2'-deoxyguanosine
-
 Anopheles gambiae
2.4.2.1 0.187
-
 guanosine
-
 Anopheles gambiae
2.4.2.1 0.19
-
 2'-deoxyinosine
-
 Anopheles gambiae
2.4.2.1 0.585
-
 Inosine
-
 Anopheles gambiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Anopheles gambiae A4Q998
-
-
2.4.2.1 Homo sapiens P00491
-
-
2.4.2.1 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Anopheles gambiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 2'-deoxyguanosine + phosphate
-
 Anopheles gambiae guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyinosine + phosphate
-
 Anopheles gambiae alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
 ?
2.4.2.1 guanosine + phosphate
-
 Anopheles gambiae guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Plasmodium falciparum hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Anopheles gambiae hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 additional information no activity is detected for adenosine or 2'-deoxyadenosine Anopheles gambiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 AgPNP
-
 Anopheles gambiae
2.4.2.1 HsPNP
-
 Homo sapiens
2.4.2.1 PfPNP
-
 Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 1
-
 guanosine
-
 Anopheles gambiae
2.4.2.1 5.4
-
 2'-deoxyguanosine
-
 Anopheles gambiae
2.4.2.1 41
-
 Inosine
-
 Anopheles gambiae
2.4.2.1 54
-
 2'-deoxyinosine
-
 Anopheles gambiae