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Literature summary extracted from

  • Ray, S.S.; Sengupta, R.; Tiso, M.; Haque, M.M.; Sahoo, R.; Konas, D.W.; Aulak, K.; Regulski, M.; Tully, T.; Stuehr, D.J.; Ghosh, S.
    Reductase domain of Drosophila melanogaster nitric-oxide synthase: redox transformations, regulation, and similarity to mammalian homologues (2007), Biochemistry, 46, 11865-11873.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 phylogenetic tree, expression of a His-tagged construct, dNOSr, comprising the dNOS reductase domain and its adjacent calmodulin binding site in Escherichia coli strain BL21 Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 additional information
-
 additional information kinetics of flavin reduction of the recombinant enzyme, overview Drosophila melanogaster
1.14.13.39 0.0032
-
 NADPH CaM-bound recombinant eNOS Drosophila melanogaster
1.14.13.39 0.0041
-
 NADPH CaM-free recombinant eNOS Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ required Drosophila melanogaster
1.14.13.39 Fe2+ required Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Drosophila melanogaster regulatory mechanism, overview 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information Drosophila melanogaster dNOS participates in essential developmental and behavioral aspects of the fruit fly ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 recombinant His-tagged construct dNOSr from Escherichia coli strain BL21 by nickel affinity and calmodulin affinity chromatography Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 additional information
-
 steady-state catalytic activities of dNOSr Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 regulatory mechanism, overview Drosophila melanogaster 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 conserved residue Arg1329 of nNOS enables bound NADPH to stabilize the FMN-shielded conformation, overview Drosophila melanogaster 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information dNOS participates in essential developmental and behavioral aspects of the fruit fly Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.39 DNOS
-
 Drosophila melanogaster
1.14.13.39 nitric-oxide synthase
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 10
-
 anaerobic pre-steady-state cytochrome c reduction assay at Drosophila melanogaster
1.14.13.39 25
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7.6
-
 assay at Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 Calmodulin
-
 Drosophila melanogaster
1.14.13.39 cytochrome c
-
 Drosophila melanogaster
1.14.13.39 FAD
-
 Drosophila melanogaster
1.14.13.39 FMN regulation of the FMN module conformational equilibrium, overview Drosophila melanogaster
1.14.13.39 NADPH dependent on Drosophila melanogaster