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Literature summary extracted from

  • Huang, V.W.; Emerson, J.P.; Kurtz, D.M.
    Reaction of Desulfovibrio vulgaris two-iron superoxide reductase with superoxide: insights from stopped-flow spectrophotometry (2007), Biochemistry, 46, 11342-11351.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.15.1.2 C13S mutant enzyme kinetics in comparison to the wild-type enzyme Desulfovibrio vulgaris
1.15.1.2 E47A the mutation of 2Fe-SOR results in an identical 600 nm intermediate that decays at the same rate as for the wild-type protein at and above neutral pH, but to a solvent- rather than glutamate-ligated resting ferric SOR site, mutant enzyme kinetics in comparison to the wild-type enzyme Desulfovibrio vulgaris
1.15.1.2 K48A mutant enzyme kinetics in comparison to the wild-type enzyme Desulfovibrio vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.2 azide
-
 Desulfovibrio vulgaris
1.15.1.2 fluoride
-
 Desulfovibrio vulgaris
1.15.1.2 formate
-
 Desulfovibrio vulgaris
1.15.1.2 phosphate
-
 Desulfovibrio vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.15.1.2 additional information
-
 additional information solvent deuterium isotope effects, stopped-flow kinetics of wild-type and mutant enzymes at different pH in different buffer systems, overview Desulfovibrio vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.2 Fe2+ two-iron superoxide reductase with a ferrous active site Desulfovibrio vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.2 reduced rubredoxin + superoxide + H+ Desulfovibrio vulgaris
-
 rubredoxin + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.2 Desulfovibrio vulgaris
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.2 reduced rubredoxin + superoxide + H+
-
 Desulfovibrio vulgaris rubredoxin + H2O2
-
 ?
1.15.1.2 reduced rubredoxin + superoxide + H+ probably via a ferric-hydroperoxo intermediate, which decays smoothly to the resting ferric active site with no other detectable intermediates, solvent proton donation occurs in the rate-determining step of dead time intermediate decay and neither of the conserved pocket residues, Glu47 or Lys48, functions as a rate-determining proton donor between pH 6.0 and pH 8.0 Desulfovibrio vulgaris rubredoxin + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.15.1.2 2Fe-SOR
-
 Desulfovibrio vulgaris
1.15.1.2 two-iron superoxide reductase
-
 Desulfovibrio vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.2 7 8 assay at Desulfovibrio vulgaris