Literature summary extracted from

Spoonamore, J.E.; Dahlgran, A.L.; Jacobsen, N.E.; Bandarian, V.
Evolution of new function in the GTP cyclohydrolase II proteins of Streptomyces coelicolor (2006), Biochemistry, 45, 12144-12155.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.5.4.25	expressed in Escherichia coli BL21(DE3) cells	Streptomyces coelicolor

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.5.4.25	additional information	the M120T variant of GCH II/II SCO 6655 acquires the ability to catalyze the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate	Streptomyces coelicolor

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.5.4.25	Zn2+	contains 1 molar equivalent of zinc per mol of protein	Streptomyces coelicolor

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.4.25	Streptomyces coelicolor	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.4.25	DEAE-Sephadex column chromatography, Q-Sepharose column chromatography, butyl-Sepharose column chromatography, and Sephacryl S-300 gel filtration	Streptomyces coelicolor

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.5.4.25	0.08	-	recombinant enzyme	Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.4.25	GTP + H2O	-	Streptomyces coelicolor	formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate	-	?
3.5.4.25	GTP + H2O	-	Streptomyces coelicolor	2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?	the reaction product is exclusively formed by enzyme variant SCO 6655	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.5.4.25	GCH II	-	Streptomyces coelicolor
3.5.4.25	GCH II/III	enzyme variant SCO 6655 shares features of GCH II and GCH III	Streptomyces coelicolor

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.5.4.25	0.028	-	GTP	recombinant enzyme	Streptomyces coelicolor

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Release 2023.1 (January 2023)