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Literature summary extracted from

  • Hoenke, S.; Wild, M.R.; Dimroth, P.
    Biosynthesis of triphosphoribosyl-dephospho-coenzyme A, the precursor of the prosthetic group of malonate decarboxylase (2000), Biochemistry, 39, 13223-13232.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.66 expression in Escherichia coli Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.66 D134A mutation abolishes the transfer of the prosthetic group to apo acyl carrier protein, but the binding of triphosphoribosyl-dephospho-CoA to MdcG is not affected Klebsiella pneumoniae
2.7.7.66 D134A/D136A mutation abolishes the transfer of the prosthetic group to apo acyl carrier protein, but the binding of triphosphoribosyl-dephospho-CoA to MdcG is not affected Klebsiella pneumoniae
2.7.7.66 D136A mutation abolishes the transfer of the prosthetic group to apo acyl carrier protein, but the binding of triphosphoribosyl-dephospho-CoA to MdcG is not affected Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.52 Klebsiella pneumoniae P71422
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2.7.7.66 Klebsiella pneumoniae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.52 ATP + 3'-dephospho-CoA
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Klebsiella pneumoniae 2'-(5-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine
-
?
2.4.2.52 additional information enzyme catalyzes starting reaction in biosynthesis of holo acyl carrier protein from the unmodified apoprotein, catalyzed by MdcB and MdcG and involving the formation of an activated precursor of the prosthetic group. In a first step, the ATP:dephospho-CoA 5ยข-triphosphoribosyl transferase MdcB catalyzes the formation of the glycosidic bond between the ribosyl moieties from ATP and dephospho-CoA, displacing the adenine moiety of ATP Klebsiella pneumoniae ?
-
?
2.7.7.66 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + malonate decarboxylase apo-[acyl-carrier protein]
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Klebsiella pneumoniae malonate decarboxylase holo-[acyl-carrier protein] + diphosphate
-
?
2.7.7.66 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + malonate decarboxylase apo-[acyl-carrier protein] MdcG forms a strong complex with the 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA prosthetic group precursor. This complex is called MdcGi. Upon incubation of MdcGi with apo acyl carrier protein, holo acyl carrier protein is synthesized by forming the phosphodiester bond between the 2'-(5''-phosphoribosyl)-3'-dephospho-CoA prosthetic group and Ser25 of the protein. In absence of the prosthetic group precursor, MdcG catalyzes at a low rate the adenylylation of apo acyl carrier protein using ATP as substrate. The adenylyl ACP thus formed is an unphysiological side product and is not involved in the biosynthesis of holo ACP Klebsiella pneumoniae malonate decarboxylase holo-[acyl-carrier protein] + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.52 MdcB
-
Klebsiella pneumoniae
2.7.7.66 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA:apo ACP 2'-(5''-phosphoribosyl)-3'-dephospho-CoA transferase
-
Klebsiella pneumoniae
2.7.7.66 MdcG
-
Klebsiella pneumoniae