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Literature summary extracted from

  • Wan, C.K.; Zhu, G.Y.; Shen, X.L.; Chattopadhyay, A.; Dey, S.; Fong, W.F.
    Gomisin A alters substrate interaction and reverses P-glycoprotein-mediated multidrug resistance in HepG2-DR cells (2006), Biochem. Pharmacol., 72, 824-837.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.2 overexpression in Hep-G2 cell Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.2 gomisin A strongly inhibits P-glycoprotein ATPase activity in cells overexpressing the enzyme. Alters substrate interaction of P-glycoprotein but itself is neither a substrate nor a competitive inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.2 0.00271
-
 verapamil/in presence of 0.4 mM gomisin A, pH 7.4, 37°C Homo sapiens
7.6.2.2 0.00426
-
 progesterone/in presence of 0.4 mM gomisin A, pH 7.4, 37°C Homo sapiens
7.6.2.2 0.00515
-
 verapamil/in pH 7.4, 37°C Homo sapiens
7.6.2.2 0.00553
-
 verapamil/in presence of 0.04 mM gomisin A, pH 7.4, 37°C Homo sapiens
7.6.2.2 0.01
-
 progesterone/in presence of 0.04 mM gomisin A, pH 7.4, 37°C Homo sapiens
7.6.2.2 0.0194
-
 progesterone/in pH 7.4, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.6.2.2 Hep-G2 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.2 ATP + H2O + progesterone/in
-
 Homo sapiens ADP + phosphate + progesterone/out
-
 ?
7.6.2.2 ATP + H2O + verapamil/in
-
 Homo sapiens ADP + phosphate + verapamil/out
-
 ?