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Literature summary extracted from

  • Taylor, M.C.; Kaur, H.; Blessington, B.; Kelly, J.M.; Wilkinson, S.R.
    Validation of spermidine synthase as a drug target in African trypanosomes (2008), Biochem. J., 409, 563-569.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.16 expression in Escherichia coli Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.16 additional information downregulation by RNAi correlates with a decrease in intracellular spermidine and cessation of growth in bloodstream form parasites. Phenotype can be rescued by expression of Leishmania major spermidine synthase gene, but not by addition of spermidine to the growth medium Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.00009
-
 S-adenosylmethioninamine pH 7.2, 37°C Trypanosoma brucei
2.5.1.16 0.205
-
 putrescine pH 7.2, 37°C Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 33000
-
 x * 33000, calculated, x * 37000, SDS-PAGE Trypanosoma brucei
2.5.1.16 37000
-
 x * 33000, calculated, x * 37000, SDS-PAGE Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Trypanosoma brucei Q38EH6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 recombinant enzyme Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 putrescine + S-adenosylmethioninamine
-
 Trypanosoma brucei spermidine + 5'-methylthioadenosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 ? x * 33000, calculated, x * 37000, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.5.1.16 SpSyn
-
 Trypanosoma brucei