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Literature summary extracted from

  • Gates, A.J.; Richardson, D.J.; Butt, J.N.
    Voltammetric characterization of the aerobic energy-dissipating nitrate reductase of Paracoccus pantotrophus: exploring the activity of a redox-balancing enzyme as a function of electrochemical potential (2008), Biochem. J., 409, 159-168.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.9.6.1 azide mixed type inhibition Paracoccus pantotrophus
1.9.6.1 thiocyanate competitive Paracoccus pantotrophus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9.6.1 0.045
-
 nitrate pH 7, NapAB catalysed nitrate reduction driven by direct electron transfer from the electrode to NapAB, protein film voltammetry Paracoccus pantotrophus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.9.6.1 16000
-
 x * 16000 + x * 90000, SDS-PAGE Paracoccus pantotrophus
1.9.6.1 90000
-
 x * 16000 + x * 90000, SDS-PAGE Paracoccus pantotrophus

Organism

EC Number Organism UniProt Comment Textmining
1.9.6.1 Paracoccus pantotrophus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.9.6.1
-
 Paracoccus pantotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.9.6.1 additional information NapAB catalysed nitrate reduction driven by direct electron transfer from the electrode to NapAB, protein film voltammetry. Exploration of the nitrate reductase activity of purified NapAB as a function of electrochemical potential, substrate concentration and pH using protein film voltammetry. Nitrate reduction by NapAB occurs at potentials below approx. 0.1 V at pH 7. These are lower potentials than required for NarGH nitrate reduction. The potentials required for Nap nitrate reduction are also likely to require ubiquinol/ubiquinone ratios higher than are needed to activate the H+-pumping oxidases expressed during aerobic growth where Nap levels are maximal. Thus the operational potentials of Paracoccus pantotrophus NapAB are consistent with a productive role in redox balancing Paracoccus pantotrophus ?
-
 ?
1.9.6.1 nitrate + reduced methyl viologen
-
 Paracoccus pantotrophus nitrite + oxidized methyl viologen + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.9.6.1 ? x * 16000 + x * 90000, SDS-PAGE Paracoccus pantotrophus

Synonyms

EC Number Synonyms Comment Organism
1.9.6.1 NapAB
-
 Paracoccus pantotrophus