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Literature summary extracted from

  • Jaemsen, J.; Tuominen, H.; Salminen, A.; Belogurov, G.A.; Magretova, N.N.; Baykov, A.A.; Lahti, R.
    A CBS domain-containing pyrophosphatase of Moorella thermoacetica is regulated by adenine nucleotides (2007), Biochem. J., 408, 327-333.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.1 ATP 1.6fold activation at 0.1 mM Moorella thermoacetica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 expression of the enzyme in Escherichia coli BL21(DE3) Moorella thermoacetica

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.1 Y169A site-directed mutagenesis of the CBS domain residue, the mutant shows altered kinetics with adenine nucleotides compared to the wild-type enzyme Moorella thermoacetica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 ADP complete inhibition at 0.1 mM Moorella thermoacetica
3.6.1.1 AMP 96% inhibition at 0.1 mM Moorella thermoacetica
3.6.1.1 cAMP 58% inhibition at 0.1 mM Moorella thermoacetica
3.6.1.1 CDP 50% inhibition at 0.1 mM Moorella thermoacetica
3.6.1.1 GDP 19% inhibition at 0.1 mM Moorella thermoacetica
3.6.1.1 GMP 18% inhibition at 0.1 mM Moorella thermoacetica
3.6.1.1 additional information poor inhibition by adenosine and UTP, no inhibition by cytidine, guanosine, uridine, CMP, CTP, GTP, cGMP, and UMP Moorella thermoacetica
3.6.1.1 UDP 35% inhibition at 0.1 mM Moorella thermoacetica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.1 additional information
-
 additional information kinetic analysis of wild-type and mutant enzymes in presence of different nucleotides, overview Moorella thermoacetica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 soluble CBS-PPase Moorella thermoacetica
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Co2+ CBS-PPase requires transition metal ions, Co2+ or Mn2+, for activity Moorella thermoacetica
3.6.1.1 Mn2+ CBS-PPase requires transition metal ions, Co2+ or Mn2+, for activity Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Moorella thermoacetica
-
 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Moorella thermoacetica
-
 strain ATCC 35608
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1 recombinant enzyme from Escherichia coli BL21(DE3) by anion exchange chromatography and gel filtration Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
 Moorella thermoacetica 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 dimer the enzyme CBS-PPase contains cystathionine beta-synthase domains Moorella thermoacetica

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 CBS-PPase
-
 Moorella thermoacetica
3.6.1.1 family II PPase
-
 Moorella thermoacetica
3.6.1.1 inorganic pyrophosphatase
-
 Moorella thermoacetica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 25
-
 assay at Moorella thermoacetica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.1 1.64
-
 diphosphate pH 7.2, 25°C Moorella thermoacetica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 7.2
-
 assay at Moorella thermoacetica