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Literature summary extracted from

  • Niyomrattanakit, P.; Yahorava, S.; Mutule, I.; Mutulis, F.; Petrovska, R.; Prusis, P.; Katzenmeier, G.; Wikberg, J.E.
    Probing the substrate specificity of the dengue virus type 2 NS3 serine protease by using internally quenched fluorescent peptides (2006), Biochem. J., 397, 203-211.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.91 additional information presence of basic residues at the P3 and P4 positions significantly promotes reactivity Dengue virus type 2

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.91 NS2B(H)-NS3pro expressed in Escherichia coli Dengue virus type 2
3.4.21.91 NS2B(H)–NS3pro expressed in Escherichia coli Dengue virus type 2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.91 0.0045
-
 Abz-RRRRSAG-3-nitrotyrosine-amide
-
 Dengue virus type 2
3.4.21.91 0.0045
-
 Abz-RRRRSAGnY-amide
-
 Dengue virus type 2
3.4.21.91 0.089
-
 Abz-KKQRAGVL-3-nitrotyrosine-amide
-
 Dengue virus type 2

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.91 Dengue virus type 2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.91
-
 Dengue virus type 2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.91 Abz-AAGRK-SLTLnY-amide + H2O
-
 Dengue virus type 2 Abz-AAGRK + SLTLnY-amide
-
 ?
3.4.21.91 Abz-AAGRKSLTL-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-AAGRK + SLTL-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-AGRK-SLTnY-amide + H2O
-
 Dengue virus type 2 Abz-AGRK + SLTnY-amide
-
 ?
3.4.21.91 Abz-AGRK-SnY-amide + H2O
-
 Dengue virus type 2 Abz-AGRK + Ser-(3-nitro)Tyr-amide
-
 ?
3.4.21.91 Abz-AGRKGTGN-3-nitrotyrosine-amide + H2O the poorest substrate Dengue virus type 2 Abz-AGRKGTGN + 3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-AGRKGTGNnY-amide + H2O
-
 Dengue virus type 2 Abz-AGRK + GTGNnY-amide
-
 ?
3.4.21.91 Abz-AGRKS-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-AGRKS + 3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-AGRKSLT-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-AGRK + SLT-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-ARRK-SLTLnY-amide + H2O
-
 Dengue virus type 2 Abz-ARRK + SLTLnY-amide
-
 ?
3.4.21.91 Abz-ARRKSLTL-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-ARRK + SLTL-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-FAAGRK-SLTLnY-amide + H2O
-
 Dengue virus type 2 Abz-FAAGRK + SLTLnY-amide
-
 ?
3.4.21.91 Abz-FAAGRKSLTL-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-FAAGRK + SLTL-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-GRK-SLTLnY-amide + H2O
-
 Dengue virus type 2 Abz-GRK + SLTLnY-amide
-
 ?
3.4.21.91 Abz-GRK-SnY-amide + H2O
-
 Dengue virus type 2 Abz-GRK + Ser-(3-nitro)Tyr-amide
-
 ?
3.4.21.91 Abz-GRKS-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-GRK + S-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-GRKSLTL-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-GRK + SLTL-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-KKQR-AGVLnY-amide + H2O high degree of cleavage efficiency, based on the NS2B/NS3 polyprotein cleavage site Dengue virus type 2 Abz-KKQR + AGVLnY-amide
-
 ?
3.4.21.91 Abz-KKQR-SAGMnY-amide + H2O
-
 Dengue virus type 2 Abz-KKQR + SAGMnY-amide
-
 ?
3.4.21.91 Abz-KKQRAGVL-3-nitrotyrosine-amide + H2O is based on the NS2B/NS3 polyprotein cleavage site, shows high degree of cleavage efficiency Dengue virus type 2 Abz-KKQR + AGVL-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-KKQRSAGM-3-nitrotyrosine-amide + H2O substrates containing the capsid protein prime side sequence SAGM are efficiently hydrolysed Dengue virus type 2 Abz-KKQR + SAGM-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-NRRRR-SAGMnYamide + H2O
-
 Dengue virus type 2 ?
-
 ?
3.4.21.91 Abz-NRRRRSAGM-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-NRRRR + SAGM-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-RK-SLTLnY-amide + H2O
-
 Dengue virus type 2 Abz-RK + SLTLnY-amide
-
 ?
3.4.21.91 Abz-RK-SnY-amide + H2O
-
 Dengue virus type 2 Abz-RK + SnY-amide
-
 ?
3.4.21.91 Abz-RKS-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-RK + S-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-RKSLTL-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-RK + SLTL-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-RRRR-3-nitrotyrosine-amide + H2O marginal activity Dengue virus type 2 Abz-RRRR + 3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-RRRR-nY-amide + H2O
-
 Dengue virus type 2 Abz-RRRR + 3-nitro-L-tyrosine amide
-
 ?
3.4.21.91 Abz-RRRR-SAGMnY-amide + H2O
-
 Dengue virus type 2 Abz-RRRR + SAGMnY-amide
-
 ?
3.4.21.91 Abz-RRRR-SAGnY-amide + H2O efficient substrate of the NS3 protease, based on the Dengue virus capsid protein processing site Dengue virus type 2 Abz-RRRR + SAGnY-amide
-
 ?
3.4.21.91 Abz-RRRR-SLTLnY-amide + H2O
-
 Dengue virus type 2 Abz-RRRR + SLTLnY-amide
-
 ?
3.4.21.91 Abz-RRRR-SnY-amide + H2O
-
 Dengue virus type 2 Abz-RRRR + Ser-(3-nitro)Tyr-amide
-
 ?
3.4.21.91 Abz-RRRRS-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-RRRR + S-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-RRRRSAG-3-nitrotyrosine-amide + H2O most efficiently hydrolysed substrate, which contains the tetrabasic non-prime side sequence RRRR of the dengue virus capsid protein Dengue virus type 2 Abz-RRRR + SAG-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-RRRRSAGM-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-RRRR + SAGM-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-RRRRSLTL-3-nitrotyrosineamide + H2O
-
 Dengue virus type 2 Abz-RRRR + SLTL-3-nitrotyrosineamide
-
 ?
3.4.21.91 Abz-SKKR-AGVLnY-amide + H2O
-
 Dengue virus type 2 Abz-SKKR + AGVLnY-amide
-
 ?
3.4.21.91 Abz-SKKR-SAGMnY-amide + H2O
-
 Dengue virus type 2 Abz-SKKR + SAGMnY-amide
-
 ?
3.4.21.91 Abz-SKKRAGVL-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-SKKR + AGVL-3-nitrotyrosine-amide
-
 ?
3.4.21.91 Abz-SKKRSAGM-3-nitrotyrosine-amide + H2O
-
 Dengue virus type 2 Abz-SKKR + SAGM-3-nitrotyrosine-amide
-
 ?
3.4.21.91 additional information preferred substrate size for the dengue virus NS3 protease encompasses the residues from P4 to P3'. The presence of basic residues at the P3 and P4 positions significantly promotes reactivity. Serine is the preferred P1' residue Dengue virus type 2 ?
-
 ?
3.4.21.91 additional information preferred substrate size for the Dengue virus NS3 protease encompasses the residues from P4 to P3'. Serine is the preferred P1' residue Dengue virus type 2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.91 dengue virus non-structural protein 3
-
 Dengue virus type 2
3.4.21.91 non-structural protein 3 serine protease
-
 Dengue virus type 2
3.4.21.91 NS3 serine protease
-
 Dengue virus type 2

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.91 0.001
-
 Abz-AGRKS-3-nitrotyrosine-amide
-
 Dengue virus type 2
3.4.21.91 0.001
-
 Abz-AGRK-SnY-amide
-
 Dengue virus type 2
3.4.21.91 0.0045
-
 Abz-SKKRAGVL-3-nitrotyrosine-amide
-
 Dengue virus type 2
3.4.21.91 0.0045
-
 Abz-SKKR-AGVLnY-amide
-
 Dengue virus type 2
3.4.21.91 0.005
-
 Abz-AGRKSLT-3-nitrotyrosine-amide
-
 Dengue virus type 2
3.4.21.91 0.005
-
 Abz-AGRK-SLTnY-amide
-
 Dengue virus type 2
3.4.21.91 0.045
-
 Abz-SKKRSAGM-3-nitrotyrosine-amide
-
 Dengue virus type 2
3.4.21.91 0.045
-
 Abz-SKKR-SAGMnY-amide
-
 Dengue virus type 2
3.4.21.91 0.0967
-
 Abz-KKQRAGVL-3-nitrotyrosine-amide
-
 Dengue virus type 2
3.4.21.91 0.0967
-
 Abz-KKQR-SAGMnY-amide
-
 Dengue virus type 2