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Literature summary extracted from

  • Bayramoglu, G.; Kiralp, S.; Yilmaz, M.; Toppare, L.; Arica, M.Y.
    Covalent immobilization of chloroperoxidase onto magnetic beads: Catalytic properties and stability (2008), Biochem. Eng. J., 38, 180-188.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.11.1.10 immobilized CPO (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads) retains 83% of its initial activity after 12 cycles of usage Leptoxyphium fumago
1.11.1.10 stability of the immobilized CPO (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads) is improved compared to free form Leptoxyphium fumago

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.10 0.276
-
 H2O2 pH 3.0, 25°C, soluble enzyme Leptoxyphium fumago
1.11.1.10 1.14
-
 H2O2 pH 3.0, 25°C, immobilized enzyme (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads) Leptoxyphium fumago

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.10 Leptoxyphium fumago
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.11.1.10 4°C, citrate buffer (50 mM, pH 3.0), 4 weeks, 91% loss of activity of the soluble enzyme, immobilized (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads) enzyme loses 15% of its initial activity Leptoxyphium fumago

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.10 monochlorodimedone + H2O2 + HCl
-
 Leptoxyphium fumago dichloromedone + H2O
-
 ?
1.11.1.10 additional information exhibits catalase, peroxidase and cytochrome P450 activities besides the halogenation reaction Leptoxyphium fumago ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.10 35
-
 soluble enzyme Leptoxyphium fumago
1.11.1.10 40
-
 immobilized enzyme (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads) Leptoxyphium fumago

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.10 20 50 20°C: about 45% of maximal activity of soluble enzyme, about 65% of maximal activity of immobilized enzyme (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads), 50°C: about 35% of maximal activity of soluble enzyme, about 65% of maximal activity of immobilized enzyme (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads) Leptoxyphium fumago

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.10 40
-
 t1/2 of free enzyme is 206.9 min Leptoxyphium fumago
1.11.1.10 50
-
 t1/2 of the free enzyme is 148.9 min, t1/2 for immobilized enzyme is 285.7 min Leptoxyphium fumago
1.11.1.10 60
-
 t1/2 of the free enzyme is 25.6 min, t1/2 for immobilized enzyme is 34.1 min Leptoxyphium fumago

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.10 2.4 3 soluble enzyme and immobilized enzyme (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads) Leptoxyphium fumago

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.10 2 4 pH 2.0: about 60% of maximal acticity of soluble enzyme, about 65% of maximal activity of immobilized enzyme (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads). pH 4.0: about 55% of maximal activity of soluble enzyme, about 75% of maximal activity of immobilized enzyme (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads) Leptoxyphium fumago