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Literature summary extracted from

  • Kataoka, K.; Tsukamoto, K.; Kitagawa, R.; Ito, T.; Sakurai, T.
    Compensatory binding of an asparagine residue to the coordination-unsaturated type I Cu center in bilirubin oxidase mutants (2008), Biochem. Biophys. Res. Commun., 371, 416-419.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.5
-
 Albifimbria verrucaria

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.5 M467F the mutated type I Cu center shows characteristics of phytocyanins Albifimbria verrucaria
1.3.3.5 M467L the mutated type I Cu center shows characteristics of phytocyanins Albifimbria verrucaria
1.3.3.5 Met467Q reduced activity Albifimbria verrucaria
1.3.3.5 N459A/M467F activity is decreased to 1% of the recombinant wild type enzyme, the mutated type I Cu center shows characteristics of phytocyanins, blue copper proteins with an axial coordination of Gln, due to compensatory binding of the distal Asn459 Albifimbria verrucaria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.5 Cu2+ contains a type I copper center Albifimbria verrucaria

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Albifimbria verrucaria
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.5 0.00041
-
 mutant enzyme M467L, using bilirubin as a substrate Albifimbria verrucaria
1.3.3.5 0.00047
-
 mutant enzyme N459A/M467F, using bilirubin as a substrate Albifimbria verrucaria
1.3.3.5 0.00064
-
 mutant enzyme M467F, using bilirubin as a substrate Albifimbria verrucaria
1.3.3.5 1.19
-
 mutant enzyme M467Q, using bilirubin as a substrate Albifimbria verrucaria
1.3.3.5 46.1
-
 recombinant wild type enzyme, using bilirubin as a substrate Albifimbria verrucaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Albifimbria verrucaria ?
-
 ?
1.3.3.5 bilirubin + O2
-
 Albifimbria verrucaria biliverdin + H2O
-
 ?
1.3.3.5 hexacyanoferrate(II) + O2
-
 Albifimbria verrucaria hexacyanoferrate(III) + H2O
-
 ?
1.3.3.5 octacyanotungstate(IV) + O2
-
 Albifimbria verrucaria octacyanotungstate(V) + H2O
-
 ?
1.3.3.5 p-phenylenediamine + O2
-
 Albifimbria verrucaria ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.5 MvBO
-
 Albifimbria verrucaria