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Literature summary extracted from
- Regulation of cuticle-degrading subtilisin proteases from the entomopathogenic fungi, Lecanicillium spp: implications for host specificity (2008), Arch. Microbiol., 189, 81-92.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.62 | chymostatin | - |
Akanthomyces muscarius |  |
| 3.4.21.62 | chymostatin | - |
Lecanicillium longisporum | |
| 3.4.21.62 | additional information | not inhibited by 1,10 phenanthroline | Akanthomyces muscarius | |
| 3.4.21.62 | additional information | not inhibited by 1,10 phenanthroline | Lecanicillium longisporum | |
| 3.4.21.62 | nitrogen | together with carbon significantly reduces protease activity of KV01 at 16 and 36 h, and KV54 at 16 h | Akanthomyces muscarius | |
| 3.4.21.62 | nitrogen | absence of C or N metabolite repression in subtilisins from KV42 isolate. Together with carbon significantly reduces protease activity of KV71 at 16 and 36 h, and KV22 at 16 h | Lecanicillium longisporum | |
| 3.4.21.62 | sucrose | together with carbon significantly reduces protease activity of KV01 at 16 and 36 h, and KV54 at 16 h | Akanthomyces muscarius | |
| 3.4.21.62 | sucrose | absence of C or N metabolite repression in subtilisins from KV42 isolate. Together with carbon significantly reduces protease activity of KV71 at 16 and 36 h, and KV22 at 16 h | Lecanicillium longisporum | |
| 3.4.21.62 | turkey egg white inhibitor | - |
Akanthomyces muscarius | |
| 3.4.21.62 | turkey egg white inhibitor | - |
Lecanicillium longisporum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.62 | Akanthomyces muscarius | - |
KV01 and KV54 | - |
| 3.4.21.62 | Lecanicillium longisporum | - |
KV71, KV22 and KV42 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.62 | Bovine serum albumin + H2O | - |
Lecanicillium longisporum | ? | - |
? | |
| 3.4.21.62 | Bovine serum albumin + H2O | - |
Akanthomyces muscarius | ? | - |
? | |
| 3.4.21.62 | chitin + H2O | - |
Lecanicillium longisporum | ? | - |
? | |
| 3.4.21.62 | chitin + H2O | - |
Akanthomyces muscarius | ? | - |
? | |
| 3.4.21.62 | cuticle + H2O | Pr1-like activity on host and non-host cuticle. In KV01 cultures, at 16 h, similar Pr1-like activity on both aphid and locust cuticle | Akanthomyces muscarius | ? | - |
? | |
| 3.4.21.62 | cuticle + H2O | Pr1-like activity on host and non-host cuticle. In KV71 cultures, at 16 and 24 h, similar Pr1-like activity on both aphid and locust cuticle. Filtrates from KV71 and KV42 cultures, that contain cocktails of cuticle-degrading enzymes, hydrolyse 3-4fold more protein from aphid than locust cuticle | Lecanicillium longisporum | ? | - |
? | |
| 3.4.21.62 | KOH chitin + H2O | - |
Lecanicillium longisporum | ? | - |
? | |
| 3.4.21.62 | KOH chitin + H2O | - |
Akanthomyces muscarius | ? | - |
? | |
| 3.4.21.62 | N-acetylglucosamine + H2O | N-acetylglucosamine has little effect on subtilisin production by KV01 (11% of that on cuticle) | Akanthomyces muscarius | ? | - |
? | |
| 3.4.21.62 | N-acetylglucosamine + H2O | restricted feeding of N-acetylglucosamine (the monomer of chitin) to biomass of KV71 induces protease to 61% of that on cuticle | Lecanicillium longisporum | ? | - |
? | |
| 3.4.21.62 | N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O | - |
Lecanicillium longisporum | ? | - |
? | |
| 3.4.21.62 | N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O | - |
Akanthomyces muscarius | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | subtilisin Pr1-like protease | - |
Lecanicillium longisporum |
| 3.4.21.62 | subtilisin Pr1-like protease | - |
Akanthomyces muscarius |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 7 | 10 | - |
Lecanicillium longisporum |
| 3.4.21.62 | 7 | 10 | - |
Akanthomyces muscarius |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.21.62 | Lecanicillium longisporum | isoelectric focusing, KV42 | - |
8 |
| 3.4.21.62 | Lecanicillium longisporum | isoelectric focusing, KV42 | - |
8.19 |
| 3.4.21.62 | Lecanicillium longisporum | isoelectric focusing, KV71 | - |
8.62 |
| 3.4.21.62 | Lecanicillium longisporum | isoelectric focusing, KV71 | - |
8.83 |
| 3.4.21.62 | Lecanicillium longisporum | isoelectric focusing, KV42 | - |
8.92 |
| 3.4.21.62 | Lecanicillium longisporum | isoelectric focusing, KV42 | - |
8.98 |
| 3.4.21.62 | Lecanicillium longisporum | isoelectric focusing, KV71 | - |
9.1 |
| 3.4.21.62 | Lecanicillium longisporum | isoelectric focusing, KV71 and KV42 | - |
9.47 |
| 3.4.21.62 | Akanthomyces muscarius | KV01 | - |
9.47 |
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