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Literature summary extracted from

  • Castro-Pinto, D.B.; Genestra, M.; Menezes, G.B.; Waghabi, M.; Goncalves, A.; De Nigris Del Cistia, C.; SantAnna, C.M.; Leon, L.L.; Mendonca-Lima, L.
    Cloning and expression of trypanothione reductase from a New World Leishmania species (2008), Arch. Microbiol., 189, 375-384.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.12 expression in Escherichia coli Leishmania amazonensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.12 53000
-
 native enzyme in promastigotes, SDS-PAGE, Western Blot Leishmania amazonensis
1.8.1.12 69200
-
 recombinant fusion protein, SDS-PAGE Leishmania amazonensis

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.12 Leishmania amazonensis Q0GU43
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.12 recombinant protein by Ni2+-charged chelating sepharose fast flow Leishmania amazonensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.12 trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid) inhibitors are tested using the recombinant enzyme Leishmania amazonensis trypanothione disulfide + thionitrobenzoate
-
 ?
1.8.1.12 trypanothione + NADP+ parasite soluble fraction Leishmania amazonensis trypanothione disulfide + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.12 trypanothione reductase TR Leishmania amazonensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.12 7.5
-
 5,5’-dithiobis-(2-nitrobenzoic acid) as substrate Leishmania amazonensis