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Literature summary extracted from

  • Hu, X.; Mamoto, R.; Shimomura, Y.; Kimbara, K.; Kawai, F.
    Cell surface structure enhancing uptake of polyvinyl alcohol (PVA) is induced by PVA in the PVA-utilizing Sphingopyxis sp. strain 113P3 (2007), Arch. Microbiol., 188, 235-241.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.30 pyrroloquinoline quinone i.e. PQQ, supplemented with polyvinyl alcohol medium at 1 mg/l Sphingopyxis sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.30 periplasm extracellular or cell-associated enzyme activity undetectable in strain 113P3 Sphingopyxis sp.
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.30 polyvinyl alcohol + O2 Sphingopyxis sp.
-
 oxidized polyvinyl alcohol + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.30 Sphingopyxis sp.
-
 reidentified from Sphingomonas sp. strain 113P3 by taxonomic analysis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.30 additional information
-
 degradation of polyvinyl alcohol measured by iodometry, polyvinyl alcohol oxidase assay, ultrastructural change on the cell surface in polyvinyl alcohol-containing media shown by Scanning electron microscopy, binding studies of fluorescein-labelled polyvinyl alcohol onto the cell surface, induction of structural surface changes by polyvinyl alcohol identifed Sphingopyxis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.30 polyvinyl alcohol + O2
-
 Sphingopyxis sp. oxidized polyvinyl alcohol + H2O2
-
 ?
1.1.3.30 polyvinyl alcohol + O2 fluorescein-labelled and unlabelled polyvinyl alcohol, degradation after uptake into the periplasm, associated with ultrastructural changes of cell surface Sphingopyxis sp. oxidized polyvinyl alcohol + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.30 30
-
 activity assay at Sphingopyxis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.30 7.2
-
 activity assay at Sphingopyxis sp.