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Literature summary extracted from

  • Ying, X.; Wang, Y.; Badiei, H.R.; Karanassios, V.; Ma, K.
    Purification and characterization of an iron-containing alcohol dehydrogenase in extremely thermophilic bacterium Thermotoga hypogea (2007), Arch. Microbiol., 187, 499-510.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.006
-
 NADPH pH 8.0, 80°C Pseudothermotoga hypogea
1.1.1.2 0.02
-
 NADP+ pH 11.0, 80°C Pseudothermotoga hypogea
1.1.1.2 0.45
-
 Butyraldehyde pH 8.0, 80°C Pseudothermotoga hypogea
1.1.1.2 1.9
-
 butanol pH 11.0, 80°C Pseudothermotoga hypogea
1.1.1.2 3.1
-
 acetaldehyde pH 8.0, 80°C Pseudothermotoga hypogea
1.1.1.2 9.7
-
 ethanol pH 11.0, 80°C Pseudothermotoga hypogea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.2 Iron a fully active enzyme contains iron of 1.02 gatoms/subunit Pseudothermotoga hypogea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 40000
-
 2 * 40000, SDS-PAGE Pseudothermotoga hypogea
1.1.1.2 70000
-
 gel filtration Pseudothermotoga hypogea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.2 aldehyde + NADPH + H+ Pseudothermotoga hypogea the physiological role of this enzyme is likely to catalyze the reduction of aldehydes to alcohols alcohol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Pseudothermotoga hypogea
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.1.2 the enzyme is oxygen sensitive. Loss of enzyme activity by exposure to oxygen could be recovered by incubation with dithiothreitol and Fe2+ Pseudothermotoga hypogea

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2
-
 Pseudothermotoga hypogea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 57
-
-
 Pseudothermotoga hypogea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 1,3-propanediol + NADP+
-
 Pseudothermotoga hypogea ?
-
 ?
1.1.1.2 1,4-butanediol + NADP+
-
 Pseudothermotoga hypogea ?
-
 ?
1.1.1.2 1,5-pentanediol + NADP+
-
 Pseudothermotoga hypogea ?
-
 ?
1.1.1.2 1-butanol + NADP+
-
 Pseudothermotoga hypogea butanal + NADPH + H+
-
 ?
1.1.1.2 1-butanol + NADP+
-
 Pseudothermotoga hypogea butyraldehyde + NADPH + H+
-
 r
1.1.1.2 1-heptanol + NADP+
-
 Pseudothermotoga hypogea heptanal + NADPH + H+
-
 ?
1.1.1.2 1-octanol + NADP+
-
 Pseudothermotoga hypogea octanal + NADPH + H+
-
 ?
1.1.1.2 1-pentanol + NADP+
-
 Pseudothermotoga hypogea n-pentanal + NADPH + H+
-
 ?
1.1.1.2 1-propanol + NADP+
-
 Pseudothermotoga hypogea ?
-
 ?
1.1.1.2 2-phenylethanol + NADP+
-
 Pseudothermotoga hypogea phenylacetaldehyde + NADPH + H+
-
 ?
1.1.1.2 aldehyde + NADPH + H+ the physiological role of this enzyme is likely to catalyze the reduction of aldehydes to alcohols Pseudothermotoga hypogea alcohol + NADP+
-
 ?
1.1.1.2 ethanol + NADP+
-
 Pseudothermotoga hypogea acetaldehyde + NADPH + H+
-
 r
1.1.1.2 hexyl alcohol + NADP+
-
 Pseudothermotoga hypogea hexanal + NADPH + H+
-
 ?
1.1.1.2 methylglyoxal + NADPH
-
 Pseudothermotoga hypogea ?
-
 ?
1.1.1.2 additional information no activity with methanol, 2-propanol, 2-butanol and acetone Pseudothermotoga hypogea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.2 dimer 2 * 40000, SDS-PAGE Pseudothermotoga hypogea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 95
-
 activity increases along with the rise of temperature up to 95°C Pseudothermotoga hypogea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 70
-
 half-life: 10 h Pseudothermotoga hypogea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.2 14
-
 ethanol pH 11.0, 80°C Pseudothermotoga hypogea
1.1.1.2 15
-
 acetaldehyde pH 8.0, 80°C Pseudothermotoga hypogea
1.1.1.2 48
-
 butanol pH 11.0, 80°C Pseudothermotoga hypogea
1.1.1.2 48
-
 Butyraldehyde pH 8.0, 80°C Pseudothermotoga hypogea
1.1.1.2 50
-
 NADP+ pH 11.0, 80°C Pseudothermotoga hypogea
1.1.1.2 52
-
 NADPH pH 8.0, 80°C Pseudothermotoga hypogea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 8
-
 production of alcohol Pseudothermotoga hypogea
1.1.1.2 11
-
 oxidation of alcohol Pseudothermotoga hypogea