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Literature summary extracted from

  • Merkens, H.; Sielker, S.; Rose, K.; Fetzner, S.
    A new monocupin quercetinase of Streptomyces sp. FLA: identification and heterologous expression of the queD gene and activity of the recombinant enzyme towards different flavonols (2007), Arch. Microbiol., 187, 475-487.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.24 overexpression in Escherichia coli or in in Streptomyces lividans TK23 Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.24 0.0141
-
 quercetin
-
 Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.24 23041
-
 x * 23041, calculated from sequence Streptomyces sp.
1.13.11.24 63000
-
 gel filtration Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.24 Streptomyces sp. A2VA43
-
-
1.13.11.24 Streptomyces sp. FLA / DSM 41951 A2VA43
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.24 recombinant QueD protein with a C-terminal hexahistidine-tag Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.24 fisetin + O2 i.e. 3,7,3',4'-tetrahydroxyflavone, 23% of the activity with quercetin Streptomyces sp. ?
-
 ?
1.13.11.24 fisetin + O2 i.e. 3,7,3',4'-tetrahydroxyflavone, 23% of the activity with quercetin Streptomyces sp. FLA / DSM 41951 ?
-
 ?
1.13.11.24 galangin + O2 i.e. 3,5,7-trihydroxyflavone, 28% of the activity with quercetin Streptomyces sp. ?
-
 ?
1.13.11.24 galangin + O2 i.e. 3,5,7-trihydroxyflavone, 28% of the activity with quercetin Streptomyces sp. FLA / DSM 41951 ?
-
 ?
1.13.11.24 kaempferol + O2 i.e. 3,5,7,4'-tetrahydroxyflavone. 70% of the activity with quercetin Streptomyces sp. ?
-
 ?
1.13.11.24 additional information no activity with luteolin Streptomyces sp. ?
-
 ?
1.13.11.24 additional information no activity with luteolin Streptomyces sp. FLA / DSM 41951 ?
-
 ?
1.13.11.24 morin + O2 i.e. 3,5,7,2',4'-pentahydroxyflavone, 1.7% of the activity with quercetin Streptomyces sp. ?
-
 ?
1.13.11.24 myricetin + O2 i.e. 3,5,7,3',4',5'-hexahydroxyflavone. 49% of the activity with quercetin Streptomyces sp. ?
-
 ?
1.13.11.24 myricetin + O2 i.e. 3,5,7,3',4',5'-hexahydroxyflavone. 49% of the activity with quercetin Streptomyces sp. FLA / DSM 41951 ?
-
 ?
1.13.11.24 quercetin + O2
-
 Streptomyces sp. 2-protocatechoylphloroglucinolcarboxylate + CO
-
 ?
1.13.11.24 quercetin + O2
-
 Streptomyces sp. FLA / DSM 41951 2-protocatechoylphloroglucinolcarboxylate + CO
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.24 ? x * 23041, calculated from sequence Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.11.24 QueD
-
 Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.24 1.45
-
 quercetin
-
 Streptomyces sp.