BRENDA - Enzyme Database

Biochemical characterization of isocitrate dehydrogenase from Methylococcus capsulatus reveals a unique NAD+-dependent homotetrameric enzyme

Stokke, R.; Madern, D.; Fedoy, A.; Karlsen, S.; Birkeland, N.; Steen, I.H.; Arch. Microbiol. 187, 361-370 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.41
cloned and overexpressed in Escherichia coli
Methylococcus capsulatus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.0517
-
isocitrate
-
Methylococcus capsulatus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
36340
-
4 * 36340, the clasp-like domain of McIDH is a likely site for tetramerization, calculated from sequence
Methylococcus capsulatus
1.1.1.41
145000
-
gel filtration
Methylococcus capsulatus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Methylococcus capsulatus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.41
-
Methylococcus capsulatus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
the enzyme is NAD+-dependent
684749
Methylococcus capsulatus
2-oxoglutarate + CO2 + NADH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.41
tetramer
4 * 36340, the clasp-like domain of McIDH is a likely site for tetramerization, calculated from sequence
Methylococcus capsulatus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.41
55
60
-
Methylococcus capsulatus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.41
70
-
apparent midpoint melting temperature
Methylococcus capsulatus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
cloned and overexpressed in Escherichia coli
Methylococcus capsulatus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.0517
-
isocitrate
-
Methylococcus capsulatus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
36340
-
4 * 36340, the clasp-like domain of McIDH is a likely site for tetramerization, calculated from sequence
Methylococcus capsulatus
1.1.1.41
145000
-
gel filtration
Methylococcus capsulatus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
-
Methylococcus capsulatus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
the enzyme is NAD+-dependent
684749
Methylococcus capsulatus
2-oxoglutarate + CO2 + NADH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.41
tetramer
4 * 36340, the clasp-like domain of McIDH is a likely site for tetramerization, calculated from sequence
Methylococcus capsulatus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.41
55
60
-
Methylococcus capsulatus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.41
70
-
apparent midpoint melting temperature
Methylococcus capsulatus