EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.18 | expressed in Escherichia coli BL21(DE3)pLysS cells | Anopheles dirus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.18 | F212A | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | F212E | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | F212H | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | F212L | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | F212W | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | F212Y | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | F216A | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | Q112A | slightly decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | R214A | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | T174A | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | Y215A | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | Y215E | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | Y215F | increased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | Y215H | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
2.5.1.18 | Y215S | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.18 | 0.01 | - |
Ethacrynic acid | mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.032 | - |
Ethacrynic acid | mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.037 | - |
Ethacrynic acid | mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.065 | - |
Ethacrynic acid | wild type enzyme, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.1 | - |
Ethacrynic acid | mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.33 | - |
glutathione | mutant enzyme T174A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.35 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215F, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.38 | - |
glutathione | mutant enzyme F212E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.44 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.45 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.48 | - |
glutathione | mutant enzyme F212Y, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.52 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme R214A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.53 | - |
glutathione | mutant enzyme F212H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.53 | - |
1-chloro-2,4-dinitrobenzene | wild type enzyme, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.6 | - |
glutathione | mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.62 | - |
glutathione | mutant enzyme R214A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.64 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.65 | - |
glutathione | mutant enzyme F212A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.67 | - |
glutathione | mutant enzyme F212W, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.7 | - |
glutathione | wild type enzyme, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.73 | - |
glutathione | mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.76 | - |
glutathione | mutant enzyme Y215E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.78 | - |
glutathione | mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.85 | - |
glutathione | mutant enzyme F216A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.85 | - |
glutathione | mutant enzyme Y215F, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.88 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.93 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.99 | - |
glutathione | mutant enzyme Y215H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 1.01 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 1.77 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212Y, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 1.78 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212W, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 1.92 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F216A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 2.03 | - |
glutathione | mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 2.35 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 2.37 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme T174A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 3.06 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 3.69 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.18 | Anopheles dirus | Q9GN60 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.18 | GSTrap affinity column chromatography | Anopheles dirus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.18 | 0.005 | - |
mutant enzyme F212H, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.005 | - |
mutant enzyme Y215E, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
2.5.1.18 | 0.006 | - |
mutant enzyme T174A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.006 | - |
mutant enzyme Y215A, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
2.5.1.18 | 0.009 | - |
mutant enzyme F212Y, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.01 | - |
mutant enzyme F212H, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.012 | - |
mutant enzyme F212Y, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.017 | - |
mutant enzyme F212W, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.017 | - |
mutant enzyme R214A, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
2.5.1.18 | 0.018 | - |
mutant enzyme Q112A, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
2.5.1.18 | 0.019 | - |
mutant enzyme Y215H, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
2.5.1.18 | 0.021 | - |
mutant enzyme F212W, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.025 | - |
mutant enzyme Y215A, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.026 | - |
mutant enzyme Y215S, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.029 | - |
mutant enzyme Y215E, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.029 | - |
mutant enzyme Y215S, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.03 | - |
mutant enzyme Y215A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.03 | - |
wild type enzyme, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
2.5.1.18 | 0.034 | - |
mutant enzyme F212A, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.035 | - |
mutant enzyme Y215E, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.036 | - |
mutant enzyme Q112A, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.036 | - |
mutant enzyme Y215A, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.04 | - |
mutant enzyme Y215H, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.041 | - |
mutant enzyme F212A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.043 | - |
mutant enzyme Y215F, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.044 | - |
mutant enzyme Q112A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.048 | - |
mutant enzyme R214A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.049 | - |
mutant enzyme Y215A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.052 | - |
mutant enzyme R214A, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.053 | - |
mutant enzyme Y215H, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.059 | - |
mutant enzyme F212L, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.068 | - |
wild type enzyme, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.069 | - |
mutant enzyme Y215F, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
2.5.1.18 | 0.073 | - |
mutant enzyme T174A, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.079 | - |
wild type enzyme, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.085 | - |
mutant enzyme F212A, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.086 | - |
mutant enzyme F212E, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.086 | - |
mutant enzyme Y215F, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
2.5.1.18 | 0.087 | - |
mutant enzyme F212L, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.12 | - |
mutant enzyme F212H, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.17 | - |
mutant enzyme Y215H, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.29 | - |
mutant enzyme Y215F, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.3 | - |
mutant enzyme Y215E, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.31 | - |
mutant enzyme F212Y, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.34 | - |
mutant enzyme F212L, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.37 | - |
mutant enzyme F212W, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.47 | - |
mutant enzyme R214A, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.47 | - |
mutant enzyme Y215A, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.51 | - |
mutant enzyme F212E, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 0.57 | - |
wild type enzyme, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.61 | - |
mutant enzyme Q112A, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 0.64 | - |
mutant enzyme Y215S, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 1.03 | - |
mutant enzyme Y215A, using ethacrynic acid as a substrate | Anopheles dirus |
2.5.1.18 | 3.03 | - |
mutant enzyme T174A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 4.28 | - |
mutant enzyme F212H, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 8.03 | - |
mutant enzyme F212Y, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 12.2 | - |
mutant enzyme F212A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 12.3 | - |
mutant enzyme F212W, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 22.9 | - |
mutant enzyme Y215A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 25.2 | - |
mutant enzyme Y215A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 26.1 | - |
mutant enzyme Y215E, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 27 | - |
mutant enzyme Y215S, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 32.2 | - |
mutant enzyme F212L, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 36.2 | - |
mutant enzyme Y215H, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 44.3 | - |
mutant enzyme R214A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 52.4 | - |
mutant enzyme Q112A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 58.6 | - |
wild type enzyme, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
2.5.1.18 | 74.3 | - |
mutant enzyme Y215F, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.18 | glutathione + 1,2-dichloro-4-nitrobenzene | weak substrate | Anopheles dirus | ? | - |
? | |
2.5.1.18 | glutathione + 1-chloro-2,4-dinitrobenzene | best substrate | Anopheles dirus | S-(2,4-dinitrophenyl)-glutathione + HCl | - |
? | |
2.5.1.18 | glutathione + 4-nitrobenzyl chloride | weak substrate | Anopheles dirus | ? | - |
? | |
2.5.1.18 | glutathione + 4-nitrophenethyl bromide | weak substrate | Anopheles dirus | ? | - |
? | |
2.5.1.18 | glutathione + ethacrynic acid | weak substrate | Anopheles dirus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.18 | GSTD4-4 | - |
Anopheles dirus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.18 | 45 | - |
the wild type enzyme shows a half-life of 13.3 min at 45°C | Anopheles dirus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.18 | 0.2 | - |
Ethacrynic acid | mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.28 | - |
Ethacrynic acid | wild type enzyme, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.36 | - |
Ethacrynic acid | mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.38 | - |
Ethacrynic acid | mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.4 | - |
Ethacrynic acid | mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 0.54 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 2.14 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme T174A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 3.55 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 6.44 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212Y, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 8.85 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212W, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 12.6 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 12.8 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F216A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 13.9 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 14.9 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 17 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 18.7 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 20.5 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme R214A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 20.7 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 26.4 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 26.5 | - |
1-chloro-2,4-dinitrobenzene | wild type enzyme, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.5.1.18 | 35.7 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215F, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus |