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Literature summary extracted from

  • Nes, W.D.; Jayasimha, P.; Song, Z.
    Yeast sterol C24-methyltransferase: role of highly conserved tyrosine-81 in catalytic competence studied by site-directed mutagenesis and thermodynamic analysis (2008), Arch. Biochem. Biophys., 477, 313-323.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.41 Y81A Km (mM): 0.018 (zymosterol), 0.023 (26,27-dehydrozymosterol), kcat (1/sec): 0.00083 (zymosterol), 0.000116 (26,27-dehydrozymosterol), Ki (mM): 0.011 (26,27-dehydrozymosterol), 0.00019 (25-azalanosterol), 0.00013 (24(R,S),25-epiminolanosterol), 0.071 (ergosterol) Saccharomyces cerevisiae
2.1.1.41 Y81F Km (mM): 0.017 (zymosterol), 0.026 (26,27-dehydrozymosterol), kcat (1/sec): 0.0108 (zymosterol), 0.0025 (26,27-dehydrozymosterol), Ki (mM): 0.010 (26,27-dehydrozymosterol), 0.00036 (25-azalanosterol), 0.00031 (24(R,S),25-epiminolanosterol), 0.082 (ergosterol) Saccharomyces cerevisiae
2.1.1.41 Y81I Km (mM): 0.021 (zymosterol), 0.027 (26,27-dehydrozymosterol), kcat (1/sec): 0.00433 (zymosterol), 0.00025 (26,27-dehydrozymosterol), Ki (mM): 0.009 (26,27-dehydrozymosterol), 0.00020 (25-azalanosterol), 0.00016 (24(R,S),25-epiminolanosterol), 0.077 (ergosterol) Saccharomyces cerevisiae
2.1.1.41 Y81L Km (mM): 0.018 (zymosterol), 0.024 (26,27-dehydrozymosterol), kcat (1/sec): 0.00416 (zymosterol), 0.00033 (26,27-dehydrozymosterol), Ki (mM): 0.011 (26,27-dehydrozymosterol), 0.00021 (25-azalanosterol), 0.00018 (24(R,S),25-epiminolanosterol), 0.078 (ergosterol) Saccharomyces cerevisiae
2.1.1.41 Y81V Km (mM): 0.026 (zymosterol), 0.024 (26,27-dehydrozymosterol), kcat (1/sec): 0.00116 (zymosterol), 0.00011 (26,27-dehydrozymosterol), Ki (mM): 0.007 (26,27-dehydrozymosterol), 0.000195 (25-azalanosterol), 0.000145 (24(R,S),25-epiminolanosterol), 0.074 (ergosterol) Saccharomyces cerevisiae
2.1.1.41 Y81W Km (mM): 0.017 (zymosterol), 0.023 (26,27-dehydrozymosterol), kcat (1/sec): 0.0133 (zymosterol), 0.0033 (26,27-dehydrozymosterol), Ki (mM): 0.010 (26,27-dehydrozymosterol), 0.00039 (25-azalanosterol), 0.00036 (24(R,S),25-epiminolanosterol), 0.088 (ergosterol) Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.41 24(R,S)-25-epiminolanosterol
-
 Saccharomyces cerevisiae
2.1.1.41 25-azalanosterol
-
 Saccharomyces cerevisiae
2.1.1.41 26,27-dehydrozymosterol
-
 Saccharomyces cerevisiae
2.1.1.41 ergosterol
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.41 0.017
-
 Zymosterol wild-type Saccharomyces cerevisiae
2.1.1.41 0.017
-
 Zymosterol mutant Y81F Saccharomyces cerevisiae
2.1.1.41 0.017
-
 Zymosterol mutant Y81W Saccharomyces cerevisiae
2.1.1.41 0.018
-
 Zymosterol mutant Y81A Saccharomyces cerevisiae
2.1.1.41 0.018
-
 Zymosterol mutant Y81L Saccharomyces cerevisiae
2.1.1.41 0.021
-
 Zymosterol mutant Y81I Saccharomyces cerevisiae
2.1.1.41 0.022
-
 26,27-dehydrozymosterol wild-type Saccharomyces cerevisiae
2.1.1.41 0.023
-
 26,27-dehydrozymosterol mutant Y81A Saccharomyces cerevisiae
2.1.1.41 0.023
-
 26,27-dehydrozymosterol mutant Y81W Saccharomyces cerevisiae
2.1.1.41 0.024
-
 26,27-dehydrozymosterol mutant Y81L Saccharomyces cerevisiae
2.1.1.41 0.024
-
 26,27-dehydrozymosterol mutant Y81V Saccharomyces cerevisiae
2.1.1.41 0.026
-
 26,27-dehydrozymosterol mutant Y81F Saccharomyces cerevisiae
2.1.1.41 0.026
-
 Zymosterol mutant Y81V Saccharomyces cerevisiae
2.1.1.41 0.027
-
 26,27-dehydrozymosterol mutant Y81I Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.41 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.41 recombinant wild-type SMT is expressed and partially purified by anionic-exchange chromatography to generate ca. 85% pure Erg6p as determined by SDS-PAGE quantification of the target protein Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.41 additional information
-
 relative activity using 26,27-dehydrozymosterol as a substrate: wild-type 100%, mutant Y81F 300%, mutant Y81W 450%, mutant Y81I 25%, mutant Y81L 40%, mutant Y81V 15%, mutant Y81A 15% Saccharomyces cerevisiae
2.1.1.41 additional information
-
 relative activity using zymosterol as a substrate: wild-type 100%, mutant Y81F 92%, mutant Y81W 124%, mutant Y81I 32%, mutant Y81L 37%, mutant Y81V 8%, mutant Y81A 8% Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.41 26,27-dehydrozymosterol + S-adenosyl-L-methionine in contrast to the results with zymosterol, product analysis of the 26,27-dehydrozymosterol assays shows that multiple products are produced that occurred in different ratios determined by HPLC-radiocounting of the non-saponifiable lipid fractions. In these analyses two major product classes are identified, a neutral C26 sterol monol and a pair of C26-oxygenated diols Saccharomyces cerevisiae ? + S-adenosyl-L-homocysteine
-
 ?
2.1.1.41 zymosterol + S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae fecosterol + S-adenosyl-L-homocysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.41 C24-methyltransferase
-
 Saccharomyces cerevisiae
2.1.1.41 Erg6p
-
 Saccharomyces cerevisiae
2.1.1.41 SMT
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.41 0.0001167
-
 26,27-dehydrozymosterol mutant Y81A Saccharomyces cerevisiae
2.1.1.41 0.0001167
-
 26,27-dehydrozymosterol mutant Y81V Saccharomyces cerevisiae
2.1.1.41 0.00025
-
 26,27-dehydrozymosterol mutant Y81I Saccharomyces cerevisiae
2.1.1.41 0.0003333
-
 26,27-dehydrozymosterol mutant Y81L Saccharomyces cerevisiae
2.1.1.41 0.0006667
-
 26,27-dehydrozymosterol wild-type Saccharomyces cerevisiae
2.1.1.41 0.0008333
-
 Zymosterol mutant Y81A Saccharomyces cerevisiae
2.1.1.41 0.001167
-
 Zymosterol mutant Y81V Saccharomyces cerevisiae
2.1.1.41 0.0025
-
 26,27-dehydrozymosterol mutant Y81F Saccharomyces cerevisiae
2.1.1.41 0.003333
-
 26,27-dehydrozymosterol mutant Y81W Saccharomyces cerevisiae
2.1.1.41 0.004167
-
 Zymosterol mutant Y81L Saccharomyces cerevisiae
2.1.1.41 0.004333
-
 Zymosterol mutant Y81I Saccharomyces cerevisiae
2.1.1.41 0.01
-
 Zymosterol mutant Y81F Saccharomyces cerevisiae
2.1.1.41 0.01083
-
 Zymosterol wild-type Saccharomyces cerevisiae
2.1.1.41 0.01333
-
 Zymosterol mutant Y81W Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.41 0.000025
-
 24(R,S)-25-epiminolanosterol wild-type Saccharomyces cerevisiae
2.1.1.41 0.00003
-
 25-azalanosterol wild-type Saccharomyces cerevisiae
2.1.1.41 0.00013
-
 24(R,S)-25-epiminolanosterol mutant Y81A Saccharomyces cerevisiae
2.1.1.41 0.000145
-
 24(R,S)-25-epiminolanosterol mutant Y81V Saccharomyces cerevisiae
2.1.1.41 0.000165
-
 24(R,S)-25-epiminolanosterol mutant Y81I Saccharomyces cerevisiae
2.1.1.41 0.00018
-
 24(R,S)-25-epiminolanosterol mutant Y81L Saccharomyces cerevisiae
2.1.1.41 0.00019
-
 25-azalanosterol mutant Y81A Saccharomyces cerevisiae
2.1.1.41 0.000195
-
 25-azalanosterol mutant Y81V Saccharomyces cerevisiae
2.1.1.41 0.000205
-
 25-azalanosterol mutant Y81I Saccharomyces cerevisiae
2.1.1.41 0.00021
-
 25-azalanosterol mutant Y81L Saccharomyces cerevisiae
2.1.1.41 0.00031
-
 24(R,S)-25-epiminolanosterol mutant Y81F Saccharomyces cerevisiae
2.1.1.41 0.00036
-
 25-azalanosterol mutant Y81F Saccharomyces cerevisiae
2.1.1.41 0.00036
-
 24(R,S)-25-epiminolanosterol mutant Y81W Saccharomyces cerevisiae
2.1.1.41 0.00039
-
 25-azalanosterol mutant Y81W Saccharomyces cerevisiae
2.1.1.41 0.00071
-
 ergosterol mutant Y81A Saccharomyces cerevisiae
2.1.1.41 0.000745
-
 ergosterol mutant Y81V Saccharomyces cerevisiae
2.1.1.41 0.00077
-
 ergosterol mutant Y81I Saccharomyces cerevisiae
2.1.1.41 0.00078
-
 ergosterol mutant Y81L Saccharomyces cerevisiae
2.1.1.41 0.00082
-
 ergosterol mutant Y81F Saccharomyces cerevisiae
2.1.1.41 0.00085
-
 ergosterol wild-type Saccharomyces cerevisiae
2.1.1.41 0.00088
-
 ergosterol mutant Y81W Saccharomyces cerevisiae
2.1.1.41 0.004
-
 26,27-dehydrozymosterol wild-type Saccharomyces cerevisiae
2.1.1.41 0.007
-
 26,27-dehydrozymosterol mutant Y81V Saccharomyces cerevisiae
2.1.1.41 0.009
-
 26,27-dehydrozymosterol mutant Y81I Saccharomyces cerevisiae
2.1.1.41 0.01
-
 26,27-dehydrozymosterol mutant Y81F Saccharomyces cerevisiae
2.1.1.41 0.01
-
 26,27-dehydrozymosterol mutant Y81W Saccharomyces cerevisiae
2.1.1.41 0.011
-
 26,27-dehydrozymosterol mutant Y81A Saccharomyces cerevisiae
2.1.1.41 0.011
-
 26,27-dehydrozymosterol mutant Y81L Saccharomyces cerevisiae