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Literature summary extracted from

  • Glatt, H.; Rost, K.; Frank, H.; Seidel, A.; Kollock, R.
    Detoxification of promutagenic aldehydes derived from methylpyrenes by human aldehyde dehydrogenases ALDH2 and ALDH3A1 (2008), Arch. Biochem. Biophys., 477, 196-205.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli BL21 (DE3) cells Homo sapiens
1.2.1.3 expressed in Escherichia coli XL-1 cells Homo sapiens
1.2.1.5 expressed in Escherichia coli XL-1 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.000027
-
 1-formyl-8-methylpyrene ALDH2, at pH 7.5 and 37°C Homo sapiens
1.2.1.3 0.00004
-
 4-formylpyrene ALDH2, at pH 7.5 and 37°C Homo sapiens
1.2.1.3 0.00015
-
 1-formyl-6-methylpyrene ALDH2, at pH 7.5 and 37°C Homo sapiens
1.2.1.3 0.00038
-
 2-formylpyrene ALDH2, at pH 7.5 and 37°C Homo sapiens
1.2.1.3 0.00048
-
 acetaldehyde ALDH2, at pH 7.5 and 37°C Homo sapiens
1.2.1.3 0.00078
-
 4-formylpyrene in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
1.2.1.3 0.0009
-
 1-formylpyrene ALDH2, at pH 7.5 and 37°C Homo sapiens
1.2.1.3 0.0016
-
 1-formyl-6-methylpyrene in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
1.2.1.3 0.004
-
 1-formylpyrene in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
1.2.1.3 0.004
-
 1-formyl-8-methylpyrene in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
1.2.1.3 0.011
-
 2-formylpyrene in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
1.2.1.3 0.23
-
 benzaldehyde in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
1.2.1.3 0.42
-
 formaldehyde ALDH2, at pH 7.5 and 37°C Homo sapiens
1.2.1.3 12
-
 propionaldehyde in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
1.2.1.3 80
-
 acetaldehyde in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
1.2.1.5 0.00078
-
 4-formylpyrene ALDH3A1, at pH 7.5 and 37°C Homo sapiens
1.2.1.5 0.0016
-
 1-formyl-6-methylpyrene ALDH3A1, at pH 7.5 and 37°C Homo sapiens
1.2.1.5 0.004
-
 1-formylpyrene ALDH3A1, at pH 7.5 and 37°C Homo sapiens
1.2.1.5 0.004
-
 1-formyl-8-methylpyrene ALDH3A1,at pH 7.5 and 37°C Homo sapiens
1.2.1.5 0.011
-
 2-formylpyrene ALDH3A1, at pH 7.5 and 37°C Homo sapiens
1.2.1.5 0.23
-
 benzaldehyde ALDH3A1, at pH 7.5 and 37°C Homo sapiens
1.2.1.5 12
-
 propionaldehyde ALDH3A1, at pH 7.5 and 37°C Homo sapiens
1.2.1.5 80
-
 acetaldehyde ALDH3A1, at pH 7.5 and 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.3 mitochondrion
-
 Homo sapiens 5739
-
1.2.1.5 mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 50390
-
 calculated from sequence of cDNA Homo sapiens
1.2.1.3 54000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Homo sapiens
-
-
-
1.2.1.3 Homo sapiens P05091
-
-
1.2.1.5 Homo sapiens P30838
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 DEAE MacroPrep column chromatography Homo sapiens
1.2.1.5 DEAE MacroPrep column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 buccal mucosa
-
 Homo sapiens
-
1.2.1.3 kidney
-
 Homo sapiens
-
1.2.1.3 liver
-
 Homo sapiens
-
1.2.1.5 buccal mucosa
-
 Homo sapiens
-
1.2.1.5 kidney
-
 Homo sapiens
-
1.2.1.5 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 1-formyl-6-methylpyrene + NAD+
-
 Homo sapiens 6-methylpyrene 1-carboxylate + NADH + H+
-
 ?
1.2.1.3 1-formyl-6-methylpyrene + NAD+ + H2O
-
 Homo sapiens 6-methylpyrene-1-carboxylic acid + NADH + H+
-
 ?
1.2.1.3 1-formyl-8-methylpyrene + NAD+ + H2O
-
 Homo sapiens 8-methylpyrene-1-carboxylic acid + NADH + H+
-
 ?
1.2.1.3 1-formyl-8-methylpyrene + NADP+
-
 Homo sapiens 8-methylpyrene 1-carboxylate + NADPH + H+
-
 ?
1.2.1.3 1-formylpyrene + NAD+
-
 Homo sapiens pyrene 1-carboxylate + NADH + H+
-
 ?
1.2.1.3 1-formylpyrene + NAD+ + H2O
-
 Homo sapiens pyrene-1-carboxylic acid + NADH + H+
-
 ?
1.2.1.3 2-formylpyrene + NAD+
-
 Homo sapiens pyrene 2-carboxylate + NADH + H+
-
 ?
1.2.1.3 2-formylpyrene + NAD+ + H2O
-
 Homo sapiens pyrene-2-carboxylic acid + NADH + H+
-
 ?
1.2.1.3 4-formylpyrene + NAD+
-
 Homo sapiens pyrene 4-carboxylate + NADH + H+
-
 ?
1.2.1.3 4-formylpyrene + NAD+ + H2O
-
 Homo sapiens pyrene-4-carboxylic acid + NADH + H+
-
 ?
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O
-
 Homo sapiens benzoate + NADH + H+
-
 ?
1.2.1.3 formaldehyde + NAD+
-
 Homo sapiens formate + NADH + H+
-
 ?
1.2.1.3 formaldehyde + NAD+ + H2O
-
 Homo sapiens formate + NADH + H+
-
 ?
1.2.1.3 additional information no activity with formaldehyde Homo sapiens ?
-
 ?
1.2.1.3 propionaldehyde + NAD+
-
 Homo sapiens propionate + NADH + H+
-
 ?
1.2.1.3 propionaldehyde + NAD+ + H2O
-
 Homo sapiens propionate + NADH + H+
-
 ?
1.2.1.5 1-formyl-6-methylpyrene + NADP+ + H2O
-
 Homo sapiens 6-methylpyrene-1-carboxylic acid + NADPH + H+
-
 ?
1.2.1.5 1-formyl-8-methylpyrene + NADP+ + H2O
-
 Homo sapiens 8-methylpyrene-1-carboxylic acid + NADPH + H+
-
 ?
1.2.1.5 1-formylpyrene + NADP+ + H2O
-
 Homo sapiens pyrene-1-carboxylic acid + NADPH + H+
-
 ?
1.2.1.5 2-formylpyrene + NADP+ + H2O
-
 Homo sapiens pyrene-2-carboxylic acid + NADPH + H+
-
 ?
1.2.1.5 4-formylpyrene + NADP+ + H2O
-
 Homo sapiens pyrene-4-carboxylic acid + NADPH + H+
-
 ?
1.2.1.5 acetaldehyde + NADP+ + H2O
-
 Homo sapiens acetate + NADPH + H+
-
 ?
1.2.1.5 benzaldehyde + NADP+ + H2O
-
 Homo sapiens benzoate + NADPH + H+
-
 ?
1.2.1.5 formaldehyde + NADP+ + H2O
-
 Homo sapiens formate + NADPH + H+
-
 ?
1.2.1.5 propionaldehyde + NADP+ + H2O
-
 Homo sapiens propionate + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 ALDH2
-
 Homo sapiens
1.2.1.3 ALDH3A1
-
 Homo sapiens
1.2.1.5 ALDH3A1
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 8.5
-
-
 Homo sapiens
1.2.1.3 9.5
-
-
 Homo sapiens
1.2.1.5 8.5
-
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.3 7.4
-
 the activity at a physiological pH value of 7.4 is nearly 50% of the activity observed at the optimal pH value Homo sapiens
1.2.1.3 7.4
-
 the activity at a physiological pH value (7.4) is nearly 40% of the activity observed at the optimal pH value Homo sapiens
1.2.1.5 7.4
-
 the activity at a physiological pH value (7.4) is nearly 50% of the activity observed at the optimal pH value Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Homo sapiens
1.2.1.5 NADP+
-
 Homo sapiens