BRENDA - Enzyme Database

Dual compartmental localization and function of mammalian NADP+-specific isocitrate dehydrogenase in yeast

Lu, Q.; Minard, K.I.; McAlister-Henn, L.; Arch. Biochem. Biophys. 472, 17-25 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
expression in Escherichia coli or Saccharomyces cerevisiae; expression of mouse isoenzyme IDP2 in Escherichia coli or Saccharomyces cerevisiae. Mouse enzyme can compensate for loss of yeast cytosolic IDP2 and of peroxisomal IDP3IDP3. Removal of the peroxisomal targeting signal of the mouse enzyme precludes both localization in peroxisomes and compensation for loss of yeast IDP3
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0037
-
NADP+
pH 7.5, isoenzyme IDP2
Mus musculus
1.1.1.42
0.0042
-
isocitrate
pH 7.5, isoenzyme IDP2
Mus musculus
1.1.1.42
0.0056
-
NADP+
pH 7.5, isoenzyme IDP1
Mus musculus
1.1.1.42
0.0058
-
isocitrate
pH 7.5, isoenzyme IDP1
Mus musculus
1.1.1.42
0.01
-
NADP+
pH 7.5, isoenzyme IDP3
Saccharomyces cerevisiae
1.1.1.42
0.015
-
isocitrate
pH 7.5, isoenzyme IDP3
Saccharomyces cerevisiae
1.1.1.42
0.02
-
NADP+
pH 7.5, isoenzyme IDP2
Saccharomyces cerevisiae
1.1.1.42
0.03
-
isocitrate
pH 7.5, isoenzyme IDP1
Saccharomyces cerevisiae
1.1.1.42
0.03
-
NADP+
pH 7.5, isoenzyme IDP1
Saccharomyces cerevisiae
1.1.1.42
0.22
-
isocitrate
pH 7.5, isoenzyme IDP2
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
mitochondrion
isoenzyme IDP1
Mus musculus
5739
-
1.1.1.42
mitochondrion
isoenzyme IDP1
Saccharomyces cerevisiae
5739
-
1.1.1.42
additional information
the mouse isoenzyme IDP2 colocalizes in cytosol and peroxisomes when expressed in yeast
Mus musculus
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Mus musculus
O88844
-
-
1.1.1.42
Mus musculus
P54071
-
-
1.1.1.42
Saccharomyces cerevisiae
P21954
-
-
1.1.1.42
Saccharomyces cerevisiae
P41939
-
-
1.1.1.42
Saccharomyces cerevisiae
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
liver
isoenzyme IDP1; isoenzyme IDP2
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
684709
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
684709
Mus musculus
2-oxoglutarate + CO2 + NADPH
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
6.5
9
more than 90% of optimal Vmax, isoenzyme IDP2
Mus musculus
1.1.1.42
7.5
-
isoenzyme IDP2
Saccharomyces cerevisiae
1.1.1.42
8
-
isoenzyme IDP1
Saccharomyces cerevisiae
1.1.1.42
8.5
-
isoenzyme IDP1
Mus musculus
1.1.1.42
8.5
-
isoenzyme IDP3
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
6.5
9
pH 6.5: about 60% of maximal activity, pH 9.0: about 70% of maximal activity, isoenzyme IDP1
Mus musculus
1.1.1.42
6.5
9
pH 6.5: about 40% of maximal activity, pH 9.0: about 70% of maximal activity, isoenzyme IDP3
Saccharomyces cerevisiae
1.1.1.42
7
8
pH 7.0: about 60% of maximal activity, pH 8.0: about 40% of maximal activity, isoenzyme IDP2
Saccharomyces cerevisiae
1.1.1.42
7
9
pH 7.0: about 60% of maximal activity, pH 9.0: about 95% of maximal activity, isoenzyme IDP1
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
expression in Escherichia coli or Saccharomyces cerevisiae
Mus musculus
1.1.1.42
expression of mouse isoenzyme IDP2 in Escherichia coli or Saccharomyces cerevisiae. Mouse enzyme can compensate for loss of yeast cytosolic IDP2 and of peroxisomal IDP3IDP3. Removal of the peroxisomal targeting signal of the mouse enzyme precludes both localization in peroxisomes and compensation for loss of yeast IDP3
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0037
-
NADP+
pH 7.5, isoenzyme IDP2
Mus musculus
1.1.1.42
0.0042
-
isocitrate
pH 7.5, isoenzyme IDP2
Mus musculus
1.1.1.42
0.0056
-
NADP+
pH 7.5, isoenzyme IDP1
Mus musculus
1.1.1.42
0.0058
-
isocitrate
pH 7.5, isoenzyme IDP1
Mus musculus
1.1.1.42
0.01
-
NADP+
pH 7.5, isoenzyme IDP3
Saccharomyces cerevisiae
1.1.1.42
0.015
-
isocitrate
pH 7.5, isoenzyme IDP3
Saccharomyces cerevisiae
1.1.1.42
0.02
-
NADP+
pH 7.5, isoenzyme IDP2
Saccharomyces cerevisiae
1.1.1.42
0.03
-
isocitrate
pH 7.5, isoenzyme IDP1
Saccharomyces cerevisiae
1.1.1.42
0.03
-
NADP+
pH 7.5, isoenzyme IDP1
Saccharomyces cerevisiae
1.1.1.42
0.22
-
isocitrate
pH 7.5, isoenzyme IDP2
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
mitochondrion
isoenzyme IDP1
Mus musculus
5739
-
1.1.1.42
mitochondrion
isoenzyme IDP1
Saccharomyces cerevisiae
5739
-
1.1.1.42
additional information
the mouse isoenzyme IDP2 colocalizes in cytosol and peroxisomes when expressed in yeast
Mus musculus
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
liver
isoenzyme IDP1
Mus musculus
-
1.1.1.42
liver
isoenzyme IDP2
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
684709
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
684709
Mus musculus
2-oxoglutarate + CO2 + NADPH
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
6.5
9
more than 90% of optimal Vmax, isoenzyme IDP2
Mus musculus
1.1.1.42
7.5
-
isoenzyme IDP2
Saccharomyces cerevisiae
1.1.1.42
8
-
isoenzyme IDP1
Saccharomyces cerevisiae
1.1.1.42
8.5
-
isoenzyme IDP1
Mus musculus
1.1.1.42
8.5
-
isoenzyme IDP3
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
6.5
9
pH 6.5: about 60% of maximal activity, pH 9.0: about 70% of maximal activity, isoenzyme IDP1
Mus musculus
1.1.1.42
6.5
9
pH 6.5: about 40% of maximal activity, pH 9.0: about 70% of maximal activity, isoenzyme IDP3
Saccharomyces cerevisiae
1.1.1.42
7
8
pH 7.0: about 60% of maximal activity, pH 8.0: about 40% of maximal activity, isoenzyme IDP2
Saccharomyces cerevisiae
1.1.1.42
7
9
pH 7.0: about 60% of maximal activity, pH 9.0: about 95% of maximal activity, isoenzyme IDP1
Saccharomyces cerevisiae