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Literature summary extracted from

  • Zheng, D.; Hrazdina, G.
    Molecular and biochemical characterization of benzalacetone synthase and chalcone synthase genes and their proteins from raspberry (Rubus idaeus L.) (2008), Arch. Biochem. Biophys., 470, 139-145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74 expression in Escherichia coli Rubus idaeus
2.3.1.212 RiPKS4, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)pLYsS Rubus idaeus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O Rubus idaeus
-
 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Rubus idaeus B0LDU5 cultivar: Royalty
-
2.3.1.74 Rubus idaeus B0LDU6 cultivar: Royalty
-
2.3.1.212 Rubus idaeus B0LDU5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.212 recombinant RiPKS4 from Escherichia coli strain BL21(DE3)pLYsS Rubus idaeus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 fruit the transcripts of RiPKS4 and RiPKS5 accumulate at a lower level during fruit development in comparison to RiPKS1 Rubus idaeus
-
2.3.1.74 leaf
-
 Rubus idaeus
-
2.3.1.212 fruit transcript of RiPKS4 accumulates at a lower level during fruit development in comparison to RiPKS1, the gene transcript is not detected during the early stage 1 for RiPKS4 of fruit development Rubus idaeus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA
-
 Rubus idaeus naringenin chalcone + 4 CoA + 3 CO2
-
 ?
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA RiPKS5 synthesizes naringenin chalcone exclusively Rubus idaeus naringenin chalcone + 4 CoA + 3 CO2
-
 ?
2.3.1.74 5-hydroxyferulyl-CoA + malonyl-CoA
-
 Rubus idaeus ? + CoA + CO2
-
 ?
2.3.1.74 caffeoyl-CoA + 3 malonyl-CoA
-
 Rubus idaeus eriodictyol + 4 CoA + 3 CO2
-
 ?
2.3.1.74 cinnamoyl-CoA + malonyl-CoA
-
 Rubus idaeus 5,7-dihydroxyflavanone + CoA + CO2
-
 ?
2.3.1.74 ferulyl-CoA + malonyl-CoA
-
 Rubus idaeus homoeriodictyol + CoA + CO2
-
 ?
2.3.1.74 sinapyl-CoA + malonyl-CoA
-
 Rubus idaeus ? + CoA + CO2
-
 ?
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
2.3.1.212 5-hydroxyferuloyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus ?
-
 ?
2.3.1.212 caffeoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus ?
-
 ?
2.3.1.212 cinnamoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus ?
-
 ?
2.3.1.212 feruloyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + (3E)-4-(4-hydroxy-3-methoxyphenyl)but-3-en-2-one + 2 CO2
-
 ?
2.3.1.212 additional information RiPKS4 is a bifunctional polyketide synthase producing both 4-hydroxybenzalacetone and naringenin chalcone, the latter by reaction of chalcone synthase, EC 2.3.1.74. The recombinant RiPKS4 protein, like the native protein from raspberry fruits accepts 4-coumaryl-CoA and ferulyl-CoA as starter substrates and catalyzes the formation of both naringenin chalcone, 4-hydroxy-benzalacetone, and 3-methoxy-4-hydroxy-benzalacetone. The activity of RiPKS4 is higher with ferulyl-CoA than with 4-coumaryl-CoA Rubus idaeus ?
-
 ?
2.3.1.212 sinapoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.74 RiPKS4
-
 Rubus idaeus
2.3.1.74 RiPKS5
-
 Rubus idaeus
2.3.1.212 RiPKS4
-
 Rubus idaeus

General Information

EC Number General Information Comment Organism
2.3.1.212 evolution the enzyme is a plant-specific type III polyketide synthase of the chalcone synthase (CHS) superfamily, amino acids C164, F215, H303 and N336 are conserved Rubus idaeus