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Literature summary extracted from

  • Vignaud, C.; Pietrancosta, N.; Williams, E.L.; Rumsby, G.; Lederer, F.
    Purification and characterization of recombinant human liver glycolate oxidase (2007), Arch. Biochem. Biophys., 465, 410-416.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.15 expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.15 2,6-dichlorophenolindophenol inhibition by excess substrate Homo sapiens
1.1.3.15 glycolate inhibition by excess substrate Homo sapiens
1.1.3.15 glyoxylate inhibition by excess substrate Homo sapiens
1.1.3.15 L-Mandelate inhibition by excess substrate Homo sapiens
1.1.3.15 Mandelate
-
 Homo sapiens
1.1.3.15 oxalate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 0.0056
-
 glycolate
-
 Homo sapiens
1.1.3.15 0.033
-
 2,6-dichlorophenolindophenol
-
 Homo sapiens
1.1.3.15 0.32
-
 glycolate
-
 Homo sapiens
1.1.3.15 1.5
-
 L-Mandelate
-
 Homo sapiens
1.1.3.15 3.4
-
 glyoxylate
-
 Homo sapiens
1.1.3.15 16.5
-
 L-lactate
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.15 41290
-
 determined by mass spectrometry. After tag-removal two close bands appear on SDS-PAGE one at 45.287 kDa and one at 41.288 kDa Homo sapiens
1.1.3.15 45290
-
 determined by mass spectrometry. After tag-removal two close bands appear on SDS-PAGE one at 45.287 kDa and one at 41.288 kDa Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.15 purified in one step on hydroxyapatite column onto which it is strongly adsorbed. For the initial purification steps, FMN is added to the buffers Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.15 liver
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
1.1.3.15 -70°C, concentrated solution, stable for several months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 glycolate + 2,6-dichlorophenolindophenol
-
 Homo sapiens glyoxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.3.15 glyoxylate + 2,6-dichlorophenolindophenol
-
 Homo sapiens ?
-
 ?
1.1.3.15 L-lactate + 2,6-dichlorophenolindophenol
-
 Homo sapiens pyruvate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.3.15 L-mandelate + 2,6-dichlorophenolindophenol
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.15 tetramer gel filtration Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.3.15 glycolate oxidase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.15 30
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.15 0.11
-
 L-Mandelate
-
 Homo sapiens
1.1.3.15 0.46
-
 L-lactate
-
 Homo sapiens
1.1.3.15 0.83
-
 glyoxylate
-
 Homo sapiens
1.1.3.15 3.6
-
 glycolate
-
 Homo sapiens
1.1.3.15 15.6
-
 glycolate
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.15 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.15 0.078
-
 2,6-dichlorophenolindophenol
-
 Homo sapiens
1.1.3.15 12
-
 glyoxylate
-
 Homo sapiens
1.1.3.15 36
-
 glycolate
-
 Homo sapiens
1.1.3.15 46
-
 glycolate
-
 Homo sapiens
1.1.3.15 92
-
 L-Mandelate
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.3.15 1.8
-
-
 Homo sapiens oxalate
1.1.3.15 3.7
-
-
 Homo sapiens Mandelate