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Literature summary extracted from

  • Wang, S.; Lee, D.P.; Gong, N.; Schwerbrock, N.M.; Mashek, D.G.; Gonzalez-Baro, M.R.; Stapleton, C.; Li, L.O.; Lewin, T.M.; Coleman, R.A.
    Cloning and functional characterization of a novel mitochondrial N-ethylmaleimide-sensitive glycerol-3-phosphate acyltransferase (GPAT2) (2007), Arch. Biochem. Biophys., 465, 347-358.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 PCR amplified mouse testis cDNA is cloned into the TOPO vector and subsequently subcloned into pcDNA3.1 for transient transfection of COS-7 and HEK293 cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 N-ethylmaleimide
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 membrane
-
 Mus musculus 16020
-
2.3.1.15 mitochondrion
-
 Mus musculus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 88800
-
 calculated molecular mass Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Mus musculus
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 to determine the translated size of GPAT2, in vitro transcription and translation is performed Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 brown adipose tissue
-
 Mus musculus
-
2.3.1.15 liver
-
 Mus musculus
-
2.3.1.15 testis
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.0002
-
 with empty pcDNA3.1 vector transfected COS-7 cells, N-ethylmaleimide resistant Mus musculus
2.3.1.15 0.0003
-
 control, untransfected COS-7 cells, N-ethylmaleimide resistant Mus musculus
2.3.1.15 0.0003
-
 with pcDNA3.1-GPAT2 transfected COS-7 cells, N-ethylmaleimide resistant Mus musculus
2.3.1.15 0.0012
-
 HEK293 cells Mus musculus
2.3.1.15 0.00149
-
 transfected HEK293 cells, 18 h after transfection Mus musculus
2.3.1.15 0.00188
-
 transfected HEK293 cells, 36 h after transfection Mus musculus
2.3.1.15 0.003
-
 with empty pcDNA3.1 vector transfected COS-7 cells, N-ethylmaleimide sensitive Mus musculus
2.3.1.15 0.0032
-
 with empty pcDNA3.1 vector transfected COS-7 cells, total Mus musculus
2.3.1.15 0.0033
-
 control, untransfected COS-7 cells, N-ethylmaleimide sensitive Mus musculus
2.3.1.15 0.0035
-
 control, untransfected COS-7 cells, total Mus musculus
2.3.1.15 0.0042
-
 with pcDNA3.1-GPAT2 transfected COS-7 cells, N-ethylmaleimide sensitive Mus musculus
2.3.1.15 0.0044
-
 with pcDNA3.1-GPAT2 transfected COS-7 cells, total Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Mus musculus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase
-
 Mus musculus
2.3.1.15 GPAT2
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 37
-
 activity assay Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 7.5
-
 activity assay Mus musculus