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Literature summary extracted from

  • Sanai, M.; Endo, S.; Matsunaga, T.; Ishikura, S.; Tajima, K.; El-Kabbani, O.; Hara, A.
    Rat NAD+-dependent 3alpha-hydroxysteroid dehydrogenase (AKR1C17): a member of the aldo-keto reductase family highly expressed in kidney cytosol (2007), Arch. Biochem. Biophys., 464, 122-129.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.50 expression of wild-type and mutant AKR1C17s in Escherichia coli strain BL21(DE3) Rattus norvegicus
1.1.1.213 expression of wild-type and mutant AKR1C17s in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.50 E276R site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKR1C9 and its cofactor specificity, overview Rattus norvegicus
1.1.1.50 Q270K site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKR1C9 and its cofactor specificity, overview Rattus norvegicus
1.1.1.50 Q270K/E276R site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKR1C9 and its cofactor specificity, overview Rattus norvegicus
1.1.1.213 E276R site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKRc9 and its cofactor specificity, overview Rattus norvegicus
1.1.1.213 Q270K site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKRc9 and its cofactor specificity, overview Rattus norvegicus
1.1.1.213 Q270K/E276R site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKRc9 and its cofactor specificity, overview Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.50 Ketamine inhibition of AKR1C17 Rattus norvegicus
1.1.1.213 4-androstene-3,17-dione versus substrate, the products, 4-androstene-3,17-dione and NADH, inhibit the activity uncompetitively and competitively, respectively, with respect to NAD+ in the presence of a saturated concentration of 0.004 mM of the substrate Rattus norvegicus
1.1.1.213 Ketamine specific inhibitor for AKR1C17, but no inhibition of AKR1C9 Rattus norvegicus
1.1.1.213 NADH the products, 4-androstene-3,17-dione and NADH, inhibit the activity uncompetitively and competitively, respectively, with respect to NAD+ in the presence of a saturated concentration of 0.004 mM of the substrate Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 additional information
-
 additional information cofactor kinetics of wild-type and mutant enzymes, overview Rattus norvegicus
1.1.1.50 0.0021
-
 5beta-androstane-3,17-dione pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.0024
-
 5beta-pregnane-3,20-dione pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.0027
-
 (5beta,20R)-20-hydroxypregnan-3-one pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.004
-
 Dehydrolithocholic acid pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.022
-
 isatin pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.023
-
 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.038
-
 9,10-phenanthrenequinone pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.42
-
 diacetyl pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 additional information
-
 additional information cofactor kinetics of wild-type and mutant enzymes, overview Rattus norvegicus
1.1.1.213 0.0021
-
 5beta-androstane-3,17-dione pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.0024
-
 5beta-pregnane-3,20-dione pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.0027
-
 (5beta,20R)-20-hydroxypregnan-3-one pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.004
-
 Dehydrolithocholic acid pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.022
-
 isatin pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.023
-
 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.038
-
 9,10-phenanthrenequinone pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.42
-
 diacetyl pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.50 cytosol AKR1C17 and AKR1C9 Rattus norvegicus 5829
-
1.1.1.50 additional information subcellular localization of isozymes, overview Rattus norvegicus
-
-
1.1.1.213 cytosol expression of AKR1C17 and AKR1C9 Rattus norvegicus 5829
-
1.1.1.213 additional information subcellular localization of isozymes, overview Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 36000
-
 recombinant AKR1C17, gel filtration Rattus norvegicus
1.1.1.50 37000
-
 1 * 37000, recombinant AKR1C17, SDS-PAGE Rattus norvegicus
1.1.1.213 36000
-
 recombinant AKR1C17, gel filtration Rattus norvegicus
1.1.1.213 37000
-
 1 * 37000, recombinant AKR1C17, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Rattus norvegicus
-
 female Wistar rats
-
1.1.1.213 Rattus norvegicus
-
 female Wistar rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50 recombinant wild-type and mutant AKR1C17s from Escherichia coli strain BL21(DE3) Rattus norvegicus
1.1.1.213 recombinant wild-type and mutant AKR1C17s from Escherichia coli strain BL21(DE3) Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.50 a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ the enzyme shows a sequential ordered reaction mechanism, where NAD+ binds to the enzyme first and NADH leaves last Rattus norvegicus
a
1.1.1.213 a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ the enzyme shows a sequential ordered reaction mechanism, where NAD+ binds to the enzyme first and NADH leaves last Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 kidney specific expression of AKR1C17, two enzymes are expressed more highly in renal cortex than in the medulla Rattus norvegicus
-
1.1.1.50 additional information tissue distribution of isozymes, overview Rattus norvegicus
-
1.1.1.213 kidney specific expression of AKR1C17, two enzymes are expressed more highly in renal cortex than in the medulla Rattus norvegicus
-
1.1.1.213 additional information tissue distribution of isozymes, overview Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 0.07
-
 recombinant AKR1C17 in cell extract, substrate 4-androsten-3alpha-ol-17-one Rattus norvegicus
1.1.1.50 0.23
-
 purified recombinant AKR1C17, substrate 4-androsten-3alpha-ol-17-one Rattus norvegicus
1.1.1.213 0.07
-
 recombinant AKR1C17 in cell extract, substrate 4-androsten-3alpha-ol-17-one Rattus norvegicus
1.1.1.213 0.23
-
 purified recombinant AKR1C17, substrate 4-androsten-3alpha-ol-17-one Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 (5beta,20R)-20-hydroxypregnan-3-one + NADH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 4-androsten-3alpha-ol-17-one + NADH + H+ AKR1C17 Rattus norvegicus 4-androsten-3alpha,17beta-diol + NAD+
-
 r
1.1.1.50 4-androstene-3,17-dione + NADH + H+ low activity Rattus norvegicus 4-androsten-3alpha-ol-17-one + NAD+
-
 r
1.1.1.50 5alpha-androstan-3alpha-ol-17-one + NADH + H+
-
 Rattus norvegicus 5beta-androstan-3alpha,17beta-diol + NAD+
-
 r
1.1.1.50 5alpha-androstane-3,17-dione + NADH low activity Rattus norvegicus 5alpha-androstan-3alpha-ol-17-one + NAD+ AKR1C9 r
1.1.1.50 5alpha-dihydrotestosterone + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.50 5alpha-pregnane-20alpha-ol-3-one + NADH
-
 Rattus norvegicus 5alpha-pregnane-3,20alpha-diol + NAD+
-
 ?
1.1.1.50 5alpha-pregnane-3,20-dione + NADH low activity Rattus norvegicus 5alpha-pregnan-3alpha-ol-20-one + NAD+
-
 r
1.1.1.50 5beta-androstan-3alpha-ol-17-one + NADH
-
 Rattus norvegicus 5beta-androstane-3alpha,17beta-diol + NAD+
-
 r
1.1.1.50 5beta-androstane-3,17-dione + NADH
-
 Rattus norvegicus 5beta-androstan-3alpha-ol-17-one + NAD+
-
 r
1.1.1.50 5beta-pregnane-20alpha-ol-3-one + NADH + H+
-
 Rattus norvegicus 5beta-pregnane-3,20alpha-diol + NAD+
-
 ?
1.1.1.50 5beta-pregnane-3,20-dione + NADH + H+
-
 Rattus norvegicus 5beta-pregnan-3alpha-ol-20-one + NAD+
-
 r
1.1.1.50 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one + NADH the enzyme produces both R- and S-form products in a ratio of 2.3 : 1 Rattus norvegicus ?
-
 ?
1.1.1.50 9,10-phenanthrenequinone + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 beta-muricholic acid + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.50 chenodeoxycholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 cholic acid + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.50 dehydrolithocholic acid + NADH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 deoxycholic acid + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.50 diacetyl + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 glycochenodeoxycholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 glycolithocholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 glycoursodeoxycholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 hyodeoxycholic acid + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.50 isatin + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 lithocholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 additional information substrate specificty, overview, recombinant AKR1C17 efficiently oxidizes 3alpha-hydroxysteroids and diverse bile acids using NAD+ as the preferred coenzyme, no activity with progesterone, cortisone, and aldosterone. The enzyme also reduces non-steroidal alpha-dicarbonyl compounds such as 9,10-phenanethrenequinone, isatin, 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one and diacetyl, but does not show significant activities towards 4-nitrobenzaldehyde and 4-nitroacetophenone Rattus norvegicus ?
-
 ?
1.1.1.50 murocholic acid + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.50 taurolithocholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 tauroursodeoxycholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.50 testosterone + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.50 ursodeoxycholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 (5beta,20R)-20-hydroxypregnan-3-one + NADH + H+
-
 Rattus norvegicus ?
-
 r
1.1.1.213 4-androsten-3alpha-ol-17-one + NADH + H+ AKR1C17 Rattus norvegicus 4-androsten-3alpha,17beta-diol + NAD+
-
 r
1.1.1.213 4-androstene-3,17-dione + NADH + H+ low activity Rattus norvegicus 4-androsten-3alpha-ol-17-one + NAD+
-
 r
1.1.1.213 5alpha-androstan-3alpha-ol-17-one + NADH + H+
-
 Rattus norvegicus 5beta-androstan-3alpha,17beta-diol + NAD+
-
 r
1.1.1.213 5alpha-androstane-3,17-dione + NADH low activity Rattus norvegicus 5alpha-androstan-3alpha-ol-17-one + NAD+ AKR1C9 r
1.1.1.213 5alpha-dihydrotestosterone + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.213 5alpha-pregnane-20alpha-ol-3-one + NADH
-
 Rattus norvegicus 5alpha-pregnane-3,20alpha-diol + NAD+
-
 ?
1.1.1.213 5alpha-pregnane-3,20-dione + NADH low activity Rattus norvegicus 5alpha-pregnan-3alpha-ol-20-one + NAD+
-
 r
1.1.1.213 5beta-androstan-3alpha-ol-17-one + NADH
-
 Rattus norvegicus 5beta-androstane-3alpha,17beta-diol + NAD+
-
 r
1.1.1.213 5beta-androstane-3,17-dione + NADH
-
 Rattus norvegicus 5beta-androstan-3alpha-ol-17-one + NAD+
-
 r
1.1.1.213 5beta-pregnane-20alpha-ol-3-one + NADH + H+
-
 Rattus norvegicus 5beta-pregnane-3,20alpha-diol + NAD+
-
 ?
1.1.1.213 5beta-pregnane-3,20-dione + NADH + H+
-
 Rattus norvegicus 5beta-pregnan-3alpha-ol-20-one + NAD+
-
 r
1.1.1.213 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one + NADH the enzyme produces both R- and S-form products in a ratio of 2.3 : 1 Rattus norvegicus ?
-
 ?
1.1.1.213 9,10-phenanthrenequinone + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 beta-muricholic acid + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.213 chenodeoxycholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 cholic acid + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.213 dehydrolithocholic acid + NADH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 deoxycholic acid + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.213 diacetyl + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 glycochenodeoxycholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 glycolithocholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 glycoursodeoxycholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 hyodeoxycholic acid + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.213 isatin + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 lithocholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 additional information substrate specificty, overview, recombinant AKR1C17 efficiently oxidizes 3alpha-hydroxysteroids and diverse bile acids using NAD+ as the preferred coenzyme, no activity with progesterone, cortisone, and aldosterone. The enzyme also reduces non-steroidal alpha-dicarbonyl compounds such as 9,10-phenanethrenequinone, isatin, 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one and diacetyl, but does not show significant activities towards 4-nitrobenzaldehyde and 4-nitroacetophenone Rattus norvegicus ?
-
 ?
1.1.1.213 murocholic acid + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.213 taurolithocholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 tauroursodeoxycholic acid + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.213 testosterone + NADH low activity Rattus norvegicus ?
-
 ?
1.1.1.213 ursodeoxycholic acid + NADH
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.50 monomer 1 * 37000, recombinant AKR1C17, SDS-PAGE Rattus norvegicus
1.1.1.213 monomer 1 * 37000, recombinant AKR1C17, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.50 AKR1C17
-
 Rattus norvegicus
1.1.1.50 AKR1C9
-
 Rattus norvegicus
1.1.1.50 More cf. EC 1.1.1.213, the enzyme belongs to the short-chain dehydrogenase/reductase family Rattus norvegicus
1.1.1.50 NAD+-dependent 3alpha-HSD
-
 Rattus norvegicus
1.1.1.50 NADP(H)-dependent 3alpha-HSD
-
 Rattus norvegicus
1.1.1.213 AKR1C17
-
 Rattus norvegicus
1.1.1.213 AKR1C9
-
 Rattus norvegicus
1.1.1.213 More cf. EC 1.1.1.50, the enzyme belongs to the short-chain dehydrogenase/reductase family Rattus norvegicus
1.1.1.213 NAD+-dependent 3alpha-HSD
-
 Rattus norvegicus
1.1.1.213 NADP(H)-dependent 3alpha-HSD
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.50 25
-
 assay at Rattus norvegicus
1.1.1.213 25
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.50 additional information
-
 additional information cofactor kinetics of wild-type and mutant enzymes, overview Rattus norvegicus
1.1.1.50 0.022
-
 Dehydrolithocholic acid pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.047
-
 5beta-androstane-3,17-dione pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.1
-
 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.105
-
 5beta-pregnane-3,20-dione pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.115
-
 (5beta,20R)-20-hydroxypregnan-3-one pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.167
-
 diacetyl pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.183
-
 9,10-phenanthrenequinone pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.50 0.283
-
 isatin pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 additional information
-
 additional information cofactor kinetics of wild-type and mutant enzymes, overview Rattus norvegicus
1.1.1.213 0.022
-
 Dehydrolithocholic acid pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.047
-
 5beta-androstane-3,17-dione pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.1
-
 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.105
-
 5beta-pregnane-3,20-dione pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.115
-
 (5beta,20R)-20-hydroxypregnan-3-one pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.167
-
 diacetyl pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.183
-
 9,10-phenanthrenequinone pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus
1.1.1.213 0.283
-
 isatin pH 7.4, 25°C, recombinant wild-type AKR1C17 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.50 7.4 9.5 recombinant enzyme Rattus norvegicus
1.1.1.213 7.4 9.5 recombinant enzyme Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.50 5.5 11
-
 Rattus norvegicus
1.1.1.213 5.5 11
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NAD+ preferred by AKR1C17, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview Rattus norvegicus
1.1.1.50 NADH
-
 Rattus norvegicus
1.1.1.50 NADP+ preferred by AKR1C9, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview Rattus norvegicus
1.1.1.50 NADPH
-
 Rattus norvegicus
1.1.1.213 NAD+ preferred by AKR1C17, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview Rattus norvegicus
1.1.1.213 NADH
-
 Rattus norvegicus
1.1.1.213 NADP+ preferred by AKR1C9, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview Rattus norvegicus
1.1.1.213 NADPH
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.50 additional information
-
 additional information inhibition kinetics, overview Rattus norvegicus
1.1.1.213 0.0022
-
 4-androstene-3,17-dione
-
 Rattus norvegicus
1.1.1.213 0.017
-
 NADH
-
 Rattus norvegicus
1.1.1.213 0.018
-
 4-androstene-3,17-dione
-
 Rattus norvegicus