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Literature summary extracted from

  • Rustler, S.; Motejadded, H.; Altenbuchner, J.; Stolz, A.
    Simultaneous expression of an arylacetonitrilase from Pseudomonas fluorescens and a (S)-oxynitrilase from Manihot esculenta in Pichia pastoris for the synthesis of (S)-mandelic acid (2008), Appl. Microbiol. Biotechnol., 80, 87-97.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.47 synthesis recombinant Pichia pastoris strains are constructed which simultaneously express the (S)-oxynitrilase of Manihot esculenta and the arylacetonitrilase of Pseudomonas fluorescens EBC191 each under the control of individual AOX1 promoters in order to obtain a whole cell catalyst for the synthesis of (S)-mandelic acid from benzaldehyde and cyanideproduction of optically active cyanohydrin compounds Manihot esculenta

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.5 expressed in Pichia pastoris Pseudomonas fluorescens
4.1.2.47 recombinant Pichia pastoris strains are constructed which simultaneously expressed the (S)-oxynitrilase of Manihot esculenta and the arylacetonitrilase of Pseudomonas fluorescens EBC191 each under the control of individual AOX1 promoters in order to obtain a whole cell catalyst for the synthesis of (S)-mandelic acid from benzaldehyde and cyanide production of optically active cyanohydrin compounds Manihot esculenta

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.5 Pseudomonas fluorescens
-
-
-
3.5.5.5 Pseudomonas fluorescens EBC191
-
-
-
4.1.2.47 Manihot esculenta
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.5 (S)-mandelonitrile + 2 H2O
-
Pseudomonas fluorescens (S)-mandelic acid + NH3
-
?
3.5.5.5 (S)-mandelonitrile + 2 H2O
-
Pseudomonas fluorescens EBC191 (S)-mandelic acid + NH3
-
?
4.1.2.47 cyanide + benzaldehyde
-
Manihot esculenta (S)-mandelonitrile
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.47 (S)-Oxynitrilase
-
Manihot esculenta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.47 5
-
-
Manihot esculenta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.5 3 7
-
Pseudomonas fluorescens
4.1.2.47 3 7
-
Manihot esculenta

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.5.5 2
-
no activity at pH 2 Pseudomonas fluorescens