EC Number | Application | Comment | Organism |
---|---|---|---|
4.1.2.47 | synthesis | recombinant Pichia pastoris strains are constructed which simultaneously express the (S)-oxynitrilase of Manihot esculenta and the arylacetonitrilase of Pseudomonas fluorescens EBC191 each under the control of individual AOX1 promoters in order to obtain a whole cell catalyst for the synthesis of (S)-mandelic acid from benzaldehyde and cyanideproduction of optically active cyanohydrin compounds | Manihot esculenta |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.5.5 | expressed in Pichia pastoris | Pseudomonas fluorescens |
4.1.2.47 | recombinant Pichia pastoris strains are constructed which simultaneously expressed the (S)-oxynitrilase of Manihot esculenta and the arylacetonitrilase of Pseudomonas fluorescens EBC191 each under the control of individual AOX1 promoters in order to obtain a whole cell catalyst for the synthesis of (S)-mandelic acid from benzaldehyde and cyanide production of optically active cyanohydrin compounds | Manihot esculenta |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.5 | Pseudomonas fluorescens | - |
- |
- |
3.5.5.5 | Pseudomonas fluorescens EBC191 | - |
- |
- |
4.1.2.47 | Manihot esculenta | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.5 | (S)-mandelonitrile + 2 H2O | - |
Pseudomonas fluorescens | (S)-mandelic acid + NH3 | - |
? | |
3.5.5.5 | (S)-mandelonitrile + 2 H2O | - |
Pseudomonas fluorescens EBC191 | (S)-mandelic acid + NH3 | - |
? | |
4.1.2.47 | cyanide + benzaldehyde | - |
Manihot esculenta | (S)-mandelonitrile | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.47 | (S)-Oxynitrilase | - |
Manihot esculenta |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.47 | 5 | - |
- |
Manihot esculenta |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 3 | 7 | - |
Pseudomonas fluorescens |
4.1.2.47 | 3 | 7 | - |
Manihot esculenta |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 2 | - |
no activity at pH 2 | Pseudomonas fluorescens |